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Gene Expression Data
Assay Details
Assay
Reference: J:10576 Rosner MH, et al., A POU-domain transcription factor in early stem cells and germ cells of the mammalian embryo. Nature. 1990 Jun 21;345(6277):686-92
Assay type: RNase protection
MGI Accession ID: MGI:3033401
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Probe: Oct3 Probe3
Probe preparation: Antisense labelled with P32 DNA
Visualized with: Autoradiography
Results
Image: 6b ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 442 bp 380 bp Amount Genetic BackgroundMutant Allele(s)Sex
F9 EC cell Cultured cells: no data stored
F9 EC cell Cultured cells: no data stored
psi2 Fibroblast Cultured cells: no data stored
Ovary P adult TS28: ovary Present Present 30 µg; total RNA Not Specified Not Specified
Spleen P adult TS28: spleen Not Specified Not Specified 30 µg; total RNA Not Specified Not Specified
Thymus P adult TS28: thymus Not Specified Present (a) 30 µg; total RNA Not Specified Not Specified
Embryo (d14) E14.0 TS22: embryo Not Specified Not Specified 30 µg; total RNA Not Specified Not Specified
Notes:
(a) Fig. 6. a shows a band for the 380 bp protected fragment in the Thymus lane that is not discussed by the authors.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory