Probe:

Msal-3 cDNA3

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Strong	Graded	2A	Expression is in a rostral to caudal gradient and strongly in the headfold.
TS11: head mesenchyme	Strong		2A
TS11: primitive streak	Present		2A	Expression is strong in the primitive streak mesoderm, but weak in the primitive streak ectoderm.

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Graded	2C	Expression is in a rostral to caudal gradient.
TS11: allantois	Present		2C
TS11: head mesenchyme	Strong	Regionally restricted	2C	Expression is in the pre-somitic mesenchyme.
TS11: primitive streak	Present		2C	Expression is strong in the primitive streak mesoderm, but weak in the primitive streak ectoderm.
TS11: embryo endoderm	Present		2C

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	2E	Expression is ventral.
TS12: neural tube	Present	Regionally restricted	2E	Expression is ventral.
TS12: head somite	Present		2E
TS12: trunk somite	Present		2E
TS12: embryo endoderm	Present		2E
TS12: embryo	Present	Regionally restricted	2E	Expression is in the tail bud.
TS12: 1st branchial arch mesenchyme derived from neural crest	Present		2E

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	2F	Expression is ventral.
TS12: neural tube	Present	Regionally restricted	2F	Expression is ventral.
TS12: head somite	Present		2F
TS12: trunk somite	Present		2F
TS12: embryo endoderm	Present		2F
TS12: embryo	Present	Regionally restricted	2F	Expression is in the tail bud.
TS12: 1st branchial arch mesenchyme derived from neural crest	Present		2F
TS12: foregut diverticulum endoderm	Present		2F
TS12: heart	Present	Regionally restricted	2F	Expression is in the heart inflow tract.

Structure	Level	Pattern	Image	Note
TS16: diencephalon	Present		3B
TS16: future hindbrain	Present	Regionally restricted	3B	Expression is at the midbrain/hindbrain boundary.
TS16: future midbrain	Present	Regionally restricted	3B	Expression is at the midbrain/hindbrain boundary.
TS16: neural tube	Present		3B
TS16: limb	Present		3B
TS16: facio-acoustic ganglion complex	Present		3B
TS16: glossopharyngeal ganglion	Present		3B
TS16: glossopharyngeal-vagus ganglion complex	Present		3B
TS16: otocyst	Present		3B
TS16: trigeminal ganglion	Present		3B
TS16: vagus ganglion	Present		3B
TS16: mesonephric tubule	Present		3B	Expression is in the pronephric and mesonephric tubules.

Structure	Level	Pattern	Image	Note
TS16: diencephalon	Strong		3D
TS16: future hindbrain	Strong		3D
TS16: future forebrain	Strong	Regionally restricted	3D	Expression is in the ventral forebrain.
TS16: otocyst	Weak	Regionally restricted	3D	Expression is in the ventral otic vesicle.
TS16: foregut	Present	Regionally restricted	3D	Expression is in the foregut endoderm.
TS16: telencephalon	Present	Regionally restricted	3D	Expression is in the dorsal-medial telencephalon.
TS16: oral region	Present	Regionally restricted	3D	Expression is in the palate.
TS16: facio-acoustic ganglion complex	Present		3D
TS16: glossopharyngeal ganglion	Present		3D
TS16: glossopharyngeal-vagus ganglion complex	Present		3D
TS16: trigeminal ganglion	Present		3D
TS16: vagus ganglion	Present		3D

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	3E	Expression is in the ventral forebrain.
TS17: future spinal cord	Present		3E
TS17: oral region	Present	Regionally restricted	3E	Expression is in the palate.
TS17: 1st branchial arch	Present		3E
TS17: 2nd branchial arch	Present		3E
TS17: 3rd branchial arch	Present		3E
TS17: 4th branchial arch	Present		3E
TS17: peripheral nervous system	Present	Regionally restricted	3E	Expression is in the peripheral nerves.
TS17: limb	Present		3E
TS17: telencephalon	Present	Regionally restricted	3E	Expression is in the dorsal-medial telencephalon.
TS17: acoustic ganglion	Present		3E
TS17: facial ganglion	Present		3E
TS17: glossopharyngeal ganglion	Present		3E
TS17: otocyst	Present		3E
TS17: trigeminal ganglion	Present		3E
TS17: vagus ganglion	Present		3E

Structure	Level	Pattern	Image	Note
TS17: mesonephric tubule	Strong		3G
TS17: neural tube floor plate	Present		3G
TS17: notochord	Absent		3G

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present		4B
TS20: alar columns	Weak	Single cells	4B	Expression is some differentiating neurons.
TS20: basal columns	Weak	Single cells	4B	Expression is some differentiating neurons.
TS20: notochord	Absent		4B

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	4D	Expression is in the intermediate and ventral ventricular zone cells.
TS20: telencephalon	Present	Regionally restricted	4D	Expression is in the dorsal-medial telencephalon.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Widespread	4F
TS20: ganglionic eminence	Present	Widespread	4F

Structure	Level	Pattern	Image	Note
TS21: medullary tubule	Strong		5C
TS21: mesenchyme of metanephros	Strong		5C
TS21: early nephron	Strong	Regionally restricted	5C	Expression is in the comma- and S-shaped bodies.

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Weak		5D

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present		6A
TS21: hindbrain	Present		6A
TS21: midbrain	Present		6A
TS21: spinal cord	Present	Regionally restricted	6A	Expression is in the ventral spinal cord.
TS21: limb	Strong	Regionally restricted	6A
TS21: mammary gland primordium	Present		6A

Structure	Level	Pattern	Image	Note
TS21: forelimb digit 1	Present	Regionally restricted	6B	Expression is in the digit tips.
TS21: forelimb digit 2	Present	Regionally restricted	6B	Expression is in the digit tips.
TS21: forelimb digit 3	Present	Regionally restricted	6B	Expression is in the digit tips.
TS21: forelimb digit 4	Present	Regionally restricted	6B	Expression is in the digit tips.
TS21: forelimb digit 5	Present	Regionally restricted	6B	Expression is in the digit tips.
TS21: forelimb interdigital region	Present		6B
TS21: forearm mesenchyme	Present	Regionally restricted	6B	Expression is in the condensing mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit 1	Present	Regionally restricted	6C	Expression is in the digit tips.
TS21: hindlimb digit 2	Present	Regionally restricted	6C	Expression is in the digit tips.
TS21: hindlimb digit 3	Present	Regionally restricted	6C	Expression is in the digit tips.
TS21: hindlimb digit 4	Present	Regionally restricted	6C	Expression is in the digit tips.
TS21: hindlimb digit 5	Present	Regionally restricted	6C	Expression is in the digit tips.
TS21: hindlimb interdigital region	Present		6C
TS21: mammary gland primordium	Present		6C