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Gene Expression Data
Assay Details
Assay
Reference: J:81847 Ivanova T, et al., Estrogen regulates tyrosine hydroxylase expression in the neonate mouse midbrain. J Neurobiol. 2003 Mar;54(4):638-47
Assay type: RT-PCR
MGI Accession ID: MGI:3029436
Gene symbol: Th
Gene name: tyrosine hydroxylase
Probe: Th-pE, Th-pF
Assay notes: Authors state that specificity of RT-PCR products was further tested by Southern blotting and hybridization with specific oligonucleotides.
Results Image: 4
 Sample Information Bands Other Sample Information
Lane AgeStructure 463 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
E15 male E15.5 (a) TS23: midbrain Present 2 µg; total RNA BALB/c Male ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
E15 female E15.5 (a) TS23: midbrain Present 2 µg; total RNA BALB/c Female ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
E17 male E17.5 (a) TS25: midbrain Present (c) 2 µg; total RNA BALB/c Male ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
E17 female E17.5 (a) TS25: midbrain Present (c) 2 µg; total RNA BALB/c Female ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
P0 male P0 TS27: midbrain Present 2 µg; total RNA BALB/c Male ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
P0 female P0 TS27: midbrain Present 2 µg; total RNA BALB/c Female ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
P7 male P7 TS28: midbrain Present (c) 2 µg; total RNA BALB/c Male ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
P7 female P7 TS28: midbrain Present (c) 2 µg; total RNA BALB/c Female ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
P15 male P15 TS28: midbrain Present 2 µg; total RNA BALB/c Male ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
P15 female P15 TS28: midbrain Present 2 µg; total RNA BALB/c Female ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
adult male P adult TS28: midbrain Present 2 µg; total RNA BALB/c Male ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
adult female P adult TS28: midbrain Present 2 µg; total RNA BALB/c Female ventral midbrain containing the substantia nigra and tegmental area, with meninges removed
Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) No statistical difference between sexes was observed.
(c) Authors report distinct expression peaks on E17 and P7.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory