About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:33825 Brown-Borg HM, et al., Developmental aspects of prolactin receptor gene expression in fetal and neonatal mice. Eur J Endocrinol. 1996 Jun;134(6):751-7
Assay type: RT-PCR
MGI Accession ID: MGI:2686999
Gene symbol: Prlr
Gene name: prolactin receptor
Probe: PR1, PR2
Visualized with: Ethidium bromide
Assay notes: The RT-PCR reaction was electrophoresed, Southern blotted, hybridized with an oligonucleotide probe (MGI:2687017) labeled with digoxigenin and visualized with chemiluminescence. The result can be seen in the lower blot of Figure 3. No obvious differences in expression were detected between the sexes at E18.5, 19.5, and P1 (other ages not specified).
Results Image: 3_upper blot
 Sample Information Bands Other Sample Information
Lane AgeStructure 698 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
Lane 1 E15.5 (a) TS23: lung Present 3 µg; total RNA involves: C3H * C57BL/6 Not Specified
Lane 2 E18.5 (a) TS26: lung Present 3 µg; total RNA involves: C3H * C57BL/6 Not Specified
Lane 3 P1 TS27: lung Present 3 µg; total RNA involves: C3H * C57BL/6 Not Specified
Lane 4 E15.5 (a) TS23: thymus primordium Weak 3 µg; total RNA involves: C3H * C57BL/6 Pooled n=3 samples pooled.
Lane 5 E18.5 (a) TS26: thymus Weak 3 µg; total RNA involves: C3H * C57BL/6 Not Specified
Lane 6 P1 TS27: thymus Weak 3 µg; total RNA involves: C3H * C57BL/6 Not Specified
Lane 7 E18.5 (a) TS26: spleen Weak 3 µg; total RNA involves: C3H * C57BL/6 Not Specified
Lane 8 E19.5 (a) TS26: spleen Weak 3 µg; total RNA involves: C3H * C57BL/6 Not Specified
Lane 9 P1 TS27: spleen Weak 3 µg; total RNA involves: C3H * C57BL/6 Not Specified
Lane 10 E18.5 (a) TS26: adrenal gland Present 3 µg; total RNA involves: C3H * C57BL/6 Pooled n=5 samples pooled.
Lane 11 E19.5 (a) TS26: adrenal gland Present 3 µg; total RNA involves: C3H * C57BL/6 Pooled n=5 samples pooled.
Lane 12 P1 TS27: adrenal gland Present 3 µg; total RNA involves: C3H * C57BL/6 Pooled n=5 samples pooled.
Lane 13 E18.5 (a) TS26: pituitary gland Strong 3 µg; total RNA involves: C3H * C57BL/6 Pooled n=5 samples pooled.
Lane 14 E19.5 (a) TS26: pituitary gland Strong 3 µg; total RNA involves: C3H * C57BL/6 Pooled n=5 samples pooled.
Lane 15 P1 TS27: pituitary gland Strong 3 µg; total RNA involves: C3H * C57BL/6 Pooled n=5 samples pooled.
Lane 16 Mol. Wt. Marker Lane
Notes:
(a) Age normalized so that noon of plug day = E0.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/30/2025
MGI 6.24 		The Jackson Laboratory