Gene Expression Data
Assay Details
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Assay
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Reference:
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J:87042
Krebs O, et al., Replicated anterior zeugopod (raz): a polydactylous mouse mutant with lowered Shh signaling in the limb bud. Development. 2003 Dec;130(24):6037-47
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2684102
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe39
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Probe preparation:
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labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A:
embryonic day 9.0
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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involves: C3H/HeJ
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
5B:
embryonic day 9.0; Raz/Raz +
(more )
Specimen
5B:
(close )
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Genetic Background:
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C3.D2-Raz
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Mutant Allele(s):
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Raz/Raz+
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
5C:
embryonic day 9.0; Raz/ Raz
(more )
Specimen
5C:
(close )
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Genetic Background:
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C3.D2-Raz
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Mutant Allele(s):
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Raz/Raz
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
5D:
embryonic day 10.25
(more )
Note: Left forelimb
Specimen
5D:
(close )
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Genetic Background:
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involves: C3H/HeJ
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Age:
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embryonic day 10.25
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left forelimb
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5D
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Expression in posterior limb mesenchyme.
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Specimen
5E:
embryonic day 10.25; Raz/Raz +
(more )
Note: Left forelimb
Specimen
5E:
(close )
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Genetic Background:
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C3.D2-Raz
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Mutant Allele(s):
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Raz/Raz+
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Age:
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embryonic day 10.25
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left forelimb
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
5E
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Expression in posterior limb mesenchyme. Authors state that the level of expression appeared downregulated compared with wt/wt
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Specimen
5F:
embryonic day 10.25; Raz/ Raz
(more )
Note: Left forelimb
Specimen
5F:
(close )
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Genetic Background:
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C3.D2-Raz
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Mutant Allele(s):
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Raz/Raz
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Age:
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embryonic day 10.25
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left forelimb
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Specimen
5G:
embryonic day 12.0
(more )
Note: Left forelimb
Specimen
5G:
(close )
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Genetic Background:
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involves: C3H/HeJ
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left forelimb
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
5G
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Expression in posterior limb mesenchyme.
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Specimen
5H:
embryonic day 12.0; Raz/Raz +
(more )
Note: Left forelimb
Specimen
5H:
(close )
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Genetic Background:
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C3.D2-Raz
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Mutant Allele(s):
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Raz/Raz+
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left forelimb
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
5H
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Expression in posterior limb mesenchyme. Authors state that the level of expression appeared downregulated compared with wt/wt
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Specimen
5I:
embryonic day 12.0; Raz/ Raz
(more )
Note: Left forelimb
Specimen
5I:
(close )
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Genetic Background:
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C3.D2-Raz
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Mutant Allele(s):
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Raz/Raz
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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Left forelimb
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Analysis Tools