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Gene Expression Data
Assay Details
Assay
Reference: J:56342 Dao D, et al., Multipoint analysis of human chromosome 11p15/mouse distal chromosome 7: inclusion of H19/IGF2 in the minimal WT2 region, gene specificity of H19 silencing in Wilms' tumorigenesis and methylation hyper-dependence of H19 imprinting. Hum Mol Genet. 1999 Jul;8(7):1337-52
Assay type: RT-PCR
MGI Accession ID: MGI:2682629
Gene symbol: Kcnq1
Gene name: potassium voltage-gated channel, subfamily Q, member 1
Probe: Kvlqt1-pG, Kvlqt1-pH
Assay notes: RT-PCR products were digested with PvuII.
Results Image: 7b Kvlqt1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Size Not Specified (c) Amount Genetic BackgroundMutant Allele(s)Sex Note
BxC, 2* E9.5 (a) TS15: embryo Absent Present 2 µg; total RNA involves: 129S4/SvJae * C57BL/6J * M. m. castaneus Not Specified Authors state that conceptus 2 is a homozygous control
BxC, 5 E9.5 (a) TS15: embryo Present Present 2 µg; total RNA involves: 129S4/SvJae * C57BL/6J * M. m. castaneus Not Specified littermate control; the genotype is either +/+ or +/-
CxB, 7 E9.5 (a) TS15: embryo Present Absent 2 µg; total RNA involves: 129S4/SvJae * C57BL/6J * M. m. castaneus Not Specified littermate control; the genotype is either +/+ or +/-
CxB, 9 E9.5 (a) TS15: embryo Present Absent 2 µg; total RNA involves: 129S4/SvJae * C57BL/6J * M. m. castaneus Not Specified littermate control; the genotype is either +/+ or +/-
BxC, 11 E8.5 (a) TS13: embryo Absent Present 2 µg; total RNA involves: 129S4/SvJae * C57BL/6J * M. m. castaneus Not Specified littermate control; the genotype is either +/+ or +/-
BxC, 12 E9.5 (a) TS15: embryo Present Present 2 µg; total RNA involves: 129S4/SvJae * C57BL/6J * M. m. castaneus Not Specified littermate control; the genotype is either +/+ or +/-
BxC, 25 E9.5 (a) TS15: embryo Present (d) Present 2 µg; total RNA involves: 129S4/SvJae * C57BL/6J * M. m. castaneus Dnmt1tm1Jae/Dnmt1tm1JaeNot Specified
BxC, 32 E9.5 (a) TS15: embryo Present (d) Present 2 µg; total RNA involves: 129S4/SvJae * C57BL/6J * M. m. castaneus Dnmt1tm1Jae/Dnmt1tm1JaeNot Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Labeled CAST
(c) Labeled BL/6
(d) Authors report partial reactivation of the imprinted allele in homozygous mutant embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory