About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:47434 Wenger RH, et al., Mouse hypoxia-inducible factor-1alpha is encoded by two different mRNA isoforms: expression from a tissue-specific and a housekeeping-type promoter. Blood. 1998 May 1;91(9):3471-80
Assay type: RT-PCR
MGI Accession ID: MGI:2680638
Gene symbol: Hif1a
Gene name: hypoxia inducible factor 1, alpha subunit
Results Image: 1_2 lanes
 Sample Information Bands Other Sample Information
Lane AgeStructure 472 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex
Brain 1 Lane not used in this assay
Brain 2 P TS28: brain Present 2.5 µg; total RNA Not Specified Not Specified
Heart 1 Lane not used in this assay
Heart 2 P TS28: heart Present 2.5 µg; total RNA Not Specified Not Specified
Lung 1 Lane not used in this assay
Lung 2 P TS28: lung Present 2.5 µg; total RNA Not Specified Not Specified
Liver 1 Lane not used in this assay
Liver 2 P TS28: liver Present 2.5 µg; total RNA Not Specified Not Specified
Kidney 1 Lane not used in this assay
Kidney 2 P TS28: metanephros Present 2.5 µg; total RNA Not Specified Not Specified
Spleen 1 Lane not used in this assay
Spleen 2 P TS28: spleen Present 2.5 µg; total RNA Not Specified Not Specified
Thymus 1 Lane not used in this assay
Thymus 2 P TS28: thymus Present 2.5 µg; total RNA Not Specified Not Specified
Bone marrow 1 Lane not used in this assay
Bone marrow 2 P TS28: bone marrow Present 2.5 µg; total RNA Not Specified Not Specified
Skeletal muscle 1 Lane not used in this assay
Skeletal muscle 2 P TS28: skeletal muscle Present 2.5 µg; total RNA Not Specified Not Specified
Tongue 1 Lane not used in this assay
Tongue 2 P TS28: tongue Present 2.5 µg; total RNA Not Specified Not Specified
Testis 1 Lane not used in this assay
Testis 2 P TS28: testis Present 2.5 µg; total RNA Not Specified Male
Ovary 1 Lane not used in this assay
Ovary 2 P TS28: ovary Present 2.5 µg; total RNA Not Specified Female
Uterus 1 Lane not used in this assay
Uterus 2 P TS28: uterus Present 2.5 µg; total RNA Not Specified Female
-RNA Control
-RNA Control
Marker Mol. Wt. Marker Lane
Notes:
(a) There is less than fourfold variation in signal intensity between the different tissues.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/18/2025
MGI 6.24
The Jackson Laboratory