Gene Expression Data
Assay Details
Assay
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Reference:
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J:79394
Ishibashi M, et al., A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development. 2002 Oct;129(20):4807-19
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2676617
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe38
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 9.0
(more )
Note: 14 somite stage.
Specimen
6A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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14 somite stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS14: future brain |
Present |
Regionally restricted |
6A
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Expressed only in ventral regions.
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Specimen
6C:
embryonic day 9.5
(more )
Note: 24 somite stage.
Specimen
6C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
|
Embryonic age was assigned based on somite numbers reported in reference.
|
Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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24 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: future brain |
Present |
Regionally restricted |
6C
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First evidence of dorsal (lateral) expansion, at the presumptive ZLI.
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Specimen
7A:
embryonic day 8.5
(more )
Note: 12 somite stage.
Specimen
7A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
|
Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
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12 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: future midbrain |
Present |
Regionally restricted |
7A
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Ventral expression domain in midbrain.
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