Probe:	Shh probe45
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	3A	Authors report expression starts at E7.5 in the midline mesoderm of the head process.
TS11: notochordal process	Present	Regionally restricted	3A	Authors report expression starts at E7.5 in the midline mesoderm of the head process.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	3B	Expression expands posteriorly along the midline into the node.
TS12: future midbrain	Present	Regionally restricted	3B	By the headfold stage, expression is initiated in the anterior midbrain of the neural ectoderm.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present		3C	Expression expands anteriorly into the ventral forebrain and posteriorly along the spinal cord.
TS13: future midbrain	Present		3C	Expression expands anteriorly into the ventral forebrain and posteriorly along the spinal cord.
TS13: future prosencephalon floor plate	Present		3C	Expression expands anteriorly into the ventral forebrain and posteriorly along the spinal cord.
TS13: future spinal cord	Present		3C	Expression expands anteriorly into the ventral forebrain and posteriorly along the spinal cord.

Structure	Level	Pattern	Image	Note
TS15: notochord	Present		3D
TS15: floor plate	Present		3D	Expression in floor plate throughout the anterior/posterior axis.
TS15: future forebrain	Present	Regionally restricted	3D	Expression along the sulcus limitans in the forebrain.
TS15: gut	Present	Regionally restricted	3D	Expression in the gut endoderm.