Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79391
Fujiwara T, et al., Distinct requirements for extra-embryonic and embryonic bone morphogenetic protein 4 in the formation of the node and primitive streak and coordination of left-right asymmetry in the mouse. Development. 2002 Oct;129(20):4685-96
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2676358
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 probe25
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 8.0
(more  )
Note: 7 somite stage.
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7 somite stage.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
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Mutant Allele(s):
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Bmp4tm1Blh/Bmp4tm1Blh
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Weak |
Regionally restricted |
2B
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Strongly reduced expression in the posterior of the embryo in 5/6 mutant embryos examined.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
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Mutant Allele(s):
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Bmp4tm1Blh/Bmp4tm1Blh
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5-6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive streak |
Present |
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Expression in prmiitive streak at a level comparable with wild-type in 1/6 mutant embryos.
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Specimen
2C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
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Mutant Allele(s):
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Bmp4tm1Blh/Bmp4tm1Blh
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Weak |
Regionally restricted |
2C
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Authors report strongly reduced expression in the posterior of the embryo.
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Specimen
5G:
embryonic day 8.0
(more )
Note: 7 somite stage.
Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7 somite stage.
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Analysis Tools
