Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:83430
Majumdar A, et al., Wnt11 and Ret/Gdnf pathways cooperate in regulating ureteric branching during metanephric kidney development. Development. 2003 Jul;130(14):3175-85
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:2675401
|
Gene symbol:
|
Ret
|
Gene name:
|
ret proto-oncogene
|
Probe:
|
Ret probe9
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
3A:
embryonic day 12.0
(more )
Specimen
3A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3A
|
Expression was detected in the 2 ampullae of the branching ureteric tips at the T-stage but is down-regulated in the stems.
|
Specimen
3A':
embryonic day 12.0
(more )
Specimen
3A':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3A'
|
Expression was detected in the ureteric tips, including the seventh ureteric tip that is seen in 42% of kidneys.
|
Specimen
3B:
embryonic day 12.25
(more )
Specimen
3B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3B
|
Expression was detected in cells at the vertices of the emerging trifurcation of the ureteric tips but is down-regulated in the stems.
|
Specimen
3B':
embryonic day 12.25
(more )
Specimen
3B':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3B'
|
Expression was detected in the ureteric tips, including the seventh ureteric tip that is seen in 42% of kidneys.
|
Specimen
3C:
embryonic day 12.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3C
|
Expression was detected in the ureteric tips but is down-regulated in the stems.
|
Specimen
3C':
embryonic day 12.5
(more )
Specimen
3C':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3C'
|
Expression was detected in the ureteric tips, including the seventh ureteric tip that is seen in 42% of kidneys.
|
Specimen
3D:
embryonic day 12.0
(more )
Specimen
3D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3D
|
Expression was detected in the 2 ampullae of the ureteric tips at the T-stage but is down-regulated in the stems.
|
Specimen
3D':
embryonic day 12.0
(more )
Specimen
3D':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3D'
|
Expression was detected in the ureteric tips, including the seventh ureteric tip that is seen in 42% of kidneys.
|
Specimen
3E:
embryonic day 12.25
(more )
Specimen
3E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3E
|
Expression was detected in cells at the vertices of the emerging trifurcation of the ureteric tips but is down-regulated in the stems.
|
Specimen
3E':
embryonic day 12.25
(more )
Specimen
3E':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3E'
|
Expression was detected in the ureteric tips, including the seventh ureteric tip that is seen in 42% of kidneys.
|
Specimen
3F:
embryonic day 12.5
(more )
Specimen
3F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3F
|
Expression was detected in the ureteric tips but was down-regulated in the stems.
|
Specimen
3F':
embryonic day 12.5
(more )
Specimen
3F':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
3F'
|
Expression was detected in the ureteric tips, including the seventh ureteric tip that is seen in 42% of kidneys.
|
Specimen
4A:
embryonic day 11.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: ureteric bud |
Present |
Regionally restricted |
4A
|
Expression was detected in the ureteric tips.
|
Specimen
4B:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Wnt11tm1Amc/Wnt11tm1Amc
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: ureteric bud |
Present |
Regionally restricted |
4B
|
Expression was detected in the ureteric tips as in wild type embryos.
|
Specimen
4E:
embryonic day 12.0
(more )
Specimen
4E:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4F:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Wnt11tm1Amc/Wnt11tm1Amc
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
4F
|
Expression was detected in the ureteric tips, but the branching process appears to be retarded in mutants.
|
Specimen
4I:
embryonic day 12.25
(more )
Specimen
4I:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Age:
|
embryonic day 12.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4J:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Wnt11tm1Amc/Wnt11tm1Amc
|
Age:
|
embryonic day 12.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
4J
|
Expression was detected in the ureteric tips, but the branching process appears to be retarded in mutants.
|
Specimen
4M:
embryonic day 12.5
(more )
Specimen
4M:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4N:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Wnt11tm1Amc/Wnt11tm1Amc
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
4N
|
Expression was detected in the ureteric tips, but the branching process appears to be retarded in mutants.
|
Specimen
6I:
embryonic day 12.5
(more )
Specimen
6I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6J:
(close )
Genetic Background:
|
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * MF1
|
Mutant Allele(s):
|
Rettm1Cos/Ret+ Wnt11tm1Amc/Wnt11+
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
6J
|
Expression was detected in the ureteric tips as in wild type embryos.
|
Specimen
6K:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Wnt11tm1Amc/Wnt11tm1Amc
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
6K
|
Expression was detected in the ureteric tips, but the branching process appears to be retarded in mutants.
|
Specimen
6L:
(close )
Genetic Background:
|
involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * MF1
|
Mutant Allele(s):
|
Rettm1Cos/Ret+ Wnt11tm1Amc/Wnt11tm1Amc
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: primitive collecting duct |
Present |
Regionally restricted |
6L
|
Expression was detected in the ureteric tips, but the branching process appears to be retarded in mutants.
|
|