Gene Expression Data
Assay Details
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Assay
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Reference:
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J:82458
Davenport TG, et al., Mammary gland, limb and yolk sac defects in mice lacking Tbx3, the gene mutated in human ulnar mammary syndrome. Development. 2003 May;130(10):2263-73
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2674224
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe35
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Assay notes:
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Some experiments may also have been performed on Swiss-Webster and ICR backgrounds. Authors state that identical phenotypes were seen on all backgrounds, and therefore all data was combined.
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Results
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Specimen
5E +/+:
embryonic day 10.5
(more  )
Specimen
5E +/+:
(close  )
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Genetic Background:
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either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129 * 129S1/Sv * 129X1/SvJ * C57BL/6NTac)
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
5E +/+
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Expression was detected in the zone of polarizing activity at the posterior margin of the fore- and hindlimb in 3 out of 4 embryos.
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| TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
5E +/+
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Expression was detected in the zone of polarizing activity at the posterior margin of the fore- and hindlimb in 3 out of 4 embryos.
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Specimen
5E -/-:
(close )
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Genetic Background:
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either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129 * 129S1/Sv * 129X1/SvJ * C57BL/6NTac)
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Mutant Allele(s):
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Tbx3tm1Pa/Tbx3tm1Pa
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Absent |
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5E -/-
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Expression was absent from the zone of polarizing activity in the posterior margin of the fore- and hindlimbs in all mutant embryos examined.
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| TS17: hindlimb bud mesenchyme |
Absent |
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5E -/-
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Expression was absent from the zone of polarizing activity in the posterior margin of the fore- and hindlimbs in all mutant embryos examined.
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Specimen
5F +/+:
embryonic day 11.5
(more )
Specimen
5F +/+:
(close )
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Genetic Background:
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either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129 * 129S1/Sv * 129X1/SvJ * C57BL/6NTac)
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Present |
Regionally restricted |
5F +/+
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Expression was detected in the zone of polarizing activity at the posterior margin of fore- and hindlimbs.
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| TS19: hindlimb bud mesenchyme |
Present |
Regionally restricted |
5F +/+
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Expression was detected in the zone of polarizing activity at the posterior margin of fore- and hindlimbs.
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Specimen
5F -/-:
(close )
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Genetic Background:
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either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129 * 129S1/Sv * 129X1/SvJ * C57BL/6NTac)
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Mutant Allele(s):
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Tbx3tm1Pa/Tbx3tm1Pa
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Weak |
Regionally restricted |
5F -/-
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In 1 of 4 mutants, expression was reduced in the zone of polarizing activity of the posterior forelimb margin.
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| TS19: hindlimb bud mesenchyme |
Weak |
Regionally restricted |
5F -/-
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In 1 of 4 mutants, expression was limited to a very small area in the zone of polarizing activity of the posterior hindlimb margin.
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Specimen
Not Shown:
(close )
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Genetic Background:
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either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129 * 129S1/Sv * 129X1/SvJ * C57BL/6NTac)
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Mutant Allele(s):
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Tbx3tm1Pa/Tbx3tm1Pa
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud mesenchyme |
Absent |
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In 3 of 4 mutants, expression was absent in the zone of polarizing activity in the posterior margin of the fore- and hindlimbs.
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| TS19: hindlimb bud mesenchyme |
Absent |
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In 3 of 4 mutants, expression was absent in the zone of polarizing activity in the posterior margin of the fore- and hindlimbs.
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Analysis Tools
