Gene Expression Data
Assay Details
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Assay
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Reference:
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J:61352
Johnson D, et al., Expression patterns of Twist and Fgfr1, -2 and -3 in the developing mouse coronal suture suggest a key role for twist in suture initiation and biogenesis. Mech Dev. 2000 Mar 1;91(1-2):341-5
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2674042
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Gene symbol:
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Twist1
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Gene name:
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twist basic helix-loop-helix transcription factor 1
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Probe:
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Twist cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 14.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: frontal bone primordium |
Present |
Regionally restricted |
1A
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Expression is throughout the subcutaneous tissue and skeletogenic membranes at the site of the developing coronal suture and in the developing parietal bone.
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| TS22: parietal bone primordium |
Present |
Regionally restricted |
1A
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Expression is throughout the subcutaneous tissue and skeletogenic membranes at the site of the developing coronal suture and in the developing parietal bone.
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| TS22: brain dura mater |
Strong |
Single cells |
1A
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Expression in some cells, presumably dura mater cells, located subjacent to the suture.
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Specimen
1E:
embryonic day 14.5
(more )
Specimen
1E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: frontal bone primordium |
Present |
Regionally restricted |
1E
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Expression is throughout the subcutaneous tissue and skeletogenic membranes at the site of the developing coronal suture.
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| TS22: parietal bone primordium |
Present |
Regionally restricted |
1E
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Expression is throughout the subcutaneous tissue and skeletogenic membranes at the site of the developing coronal suture.
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Specimen
2A:
embryonic day 16.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: frontal bone |
Present |
Regionally restricted |
2A
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Expression is in the outermost cells of the developing bone plates.
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| TS24: parietal bone |
Present |
Regionally restricted |
2A
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Expression is in the outermost cells of the developing bone plates.
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Specimen
2E:
embryonic day 16.5
(more )
Specimen
2E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: frontal bone |
Present |
Regionally restricted |
2E
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Expression is in the outermost cells of the developing bone plates.
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| TS24: parietal bone |
Present |
Regionally restricted |
2E
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Expression is in the outermost cells of the developing bone plates.
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Specimen
Not Shown:
embryonic day 17.5
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
3A:
embryonic day 18.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Specimen
3E:
embryonic day 18.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Analysis Tools