Gene Expression Data
Assay Details
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Assay
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Reference:
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J:82459
Laclef C, et al., Altered myogenesis in Six1-deficient mice. Development. 2003 May;130(10):2239-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2673283
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Antibody:
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Anti-Pax3
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Detection system:
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Secondary antibody
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Assay notes:
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No expression differences were noted between wild type, heterozygous and homozygous mutant littermates with this assay. Identical results were obtained with comparison of the mutation on the 129/Sv and C57BL/6 strain backgrounds.
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Results
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Specimen
5C:
(close  )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6)
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5D:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6)
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk dermomyotome |
Present |
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5D -/-
,
5E -/-
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Expression was detected in dermomyotome and migrating myogenic cells delaminating from the lateral edge of the dermomyotome at forelimb level.
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Specimen
5G:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6)
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud mesenchyme |
Present |
Regionally restricted |
5G -/-
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Expression was detected in the ventral and dorsal migrating myogenic cells.
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Specimen
Not Shown:
embryonic day 9.5
(more  )
Specimen
Not Shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * C57BL/6)
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Not Shown:
embryonic day 9.5; Six1tm1Mair/Six1 +
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * C57BL/6)
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Mutant Allele(s):
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Six1tm1Mair/Six1+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Not Shown:
embryonic day 11.0
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * C57BL/6)
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk dermomyotome |
Present |
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|
Expression was detected in dermomyotome and migrating myogenic cells delaminating from the lateral edge of the dermomyotome at forelimb level.
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| TS18: forelimb bud mesenchyme |
Present |
Regionally restricted |
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Expression was detected in the ventral and dorsal migrating myogenic cells.
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Specimen
Not Shown:
embryonic day 11.0; Six1tm1Mair/Six1 +
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * C57BL/6)
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Mutant Allele(s):
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Six1tm1Mair/Six1+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk dermomyotome |
Present |
|
|
Expression was detected in dermomyotome and migrating myogenic cells delaminating from the lateral edge of the dermomyotome at forelimb level.
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| TS18: forelimb bud mesenchyme |
Present |
Regionally restricted |
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Expression was detected in the ventral and dorsal migrating myogenic cells.
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Analysis Tools
