Gene Expression Data
Assay Details
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Assay
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Reference:
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J:82459
Laclef C, et al., Altered myogenesis in Six1-deficient mice. Development. 2003 May;130(10):2239-52
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2673282
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Antibody:
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Anti-Myf-5
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Detection system:
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Secondary antibody
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Assay notes:
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No expression differences were noted between wild type, heterozygous and homozygous mutant littermates with this assay. Identical results were obtained with comparison of the mutation on the 129/Sv and C57BL/6 strain backgrounds.
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Results
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Specimen
5A:
(close  )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6)
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5H:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6)
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5I:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6)
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Mutant Allele(s):
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Six1tm1Mair/Six1tm1Mair
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Not Shown:
embryonic day 9.5
(more  )
Specimen
Not Shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * C57BL/6)
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk dermomyotome |
Present |
Regionally restricted |
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Expression was detected in the myotome.
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Specimen
Not Shown:
embryonic day 9.5; Six1tm1Mair/Six1 +
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * C57BL/6)
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Mutant Allele(s):
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Six1tm1Mair/Six1+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: trunk dermomyotome |
Present |
Regionally restricted |
|
Expression was detected in the myotome.
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Specimen
Not Shown:
embryonic day 11.0
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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either: (involves: 129) or (involves: 129 * C57BL/6)
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Not Shown:
embryonic day 11.0; Six1tm1Mair/Six1 +
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
|
either: (involves: 129) or (involves: 129 * C57BL/6)
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Mutant Allele(s):
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Six1tm1Mair/Six1+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools
