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Gene Expression Data
Assay Details
Assay
Reference: J:44959 Watrin F, et al., The mouse Necdin gene is expressed from the paternal allele only and lies in the 7C region of the mouse chromosome 7, a region of conserved synteny to the human Prader-Willi syndrome region. Eur J Hum Genet. 1997 Sep-Oct;5(5):324-32
Assay type: RT-PCR
MGI Accession ID: MGI:2667876
Gene symbol: Ndn
Gene name: necdin, MAGE family member
Probe: Nec-1859, Nec-2420
Probe preparation: DNA
Visualized with: Ethidium bromide
Results Image: 1c
 Sample Information Bands Other Sample Information
Lane AgeStructure 561 bp (a) 561 bp (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
TR Mol. Wt. Marker Lane
B - Control
B + P adult TS28: brain Not Applicable Present 2 µg; total RNA C57BL/6 Not Specified
S - Control
S + P adult TS28: brain Present Not Applicable 2 µg; total RNA M. spretus Not Specified
(BXS)F1 - Control
(BXS)F1 + P adult TS28: brain Present Absent 2 µg; total RNA (C57BL/6 x M. spretus)F1 Not Specified
(BXS)F1 - Control
(BXS)F1 + P adult TS28: brain Present Absent 2 µg; total RNA (C57BL/6 x M. spretus)F1 Not Specified
[(BXS)XB]N2 - Control
[(BXS)XB]N2 + P adult TS28: brain Absent Present 2 µg; total RNA involves: C57BL/6 * M. spretus Not Specified [(C57BL/6 X M. spretus) X C57BL/6]N2
[(BXS)XB]N2 - Control
[(BXS)XB]N2 + P adult TS28: brain Absent Present 2 µg; total RNA involves: C57BL/6 * M. spretus Not Specified [(C57BL/6 X M. spretus) X C57BL/6]N2
(BXS) 12.5D EB - Control
(BXS) 12.5D EB + E12.5 (c) TS20: head Present Absent 2 µg; total RNA (C57BL/6 x M. spretus)F1 Not Specified
Lane 16 Mol. Wt. Marker Lane
Notes:
(a) This band represents the M. spretus product.
(b) This band represents the C57BL/6 product. It is susceptible to digestion with TaqI; it cleaves into 399 and 162 bp pieces.
(c) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory