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Gene Expression Data
Assay Details
Assay
Reference: J:19085 Lewis SA, et al., Five mouse tubulin isotypes and their regulated expression during development. J Cell Biol. 1985 Sep;101(3):852-61
Assay type: Northern blot
MGI Accession ID: MGI:2667851
Gene symbol: Tubb5
Gene name: tubulin, beta 5 class I
Probe: M-beta-5 probe1
Probe preparation: Double stranded labelled with P32 DNA
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 2.8 kb (a) 1.8 kb (a) Amount Genetic BackgroundMutant Allele(s)Sex
Lane 1 P10 TS28: musculature Present Present 10 µg; total RNA Not Specified Male
Lane 2 P15 TS28: musculature Present Present 10 µg; total RNA Not Specified Male
Lane 3 P22 TS28: musculature Absent Absent 10 µg; total RNA Not Specified Male
Lane 4 P32 TS28: musculature Absent Absent 10 µg; total RNA Not Specified Male
Lane 5 P3 TS27: spleen Present Present 10 µg; total RNA Not Specified Not Specified
Lane 6 P6 TS28: spleen Present Present 10 µg; total RNA Not Specified Not Specified
Lane 7 P10 TS28: spleen Present Present 10 µg; total RNA Not Specified Not Specified
Lane 8 P15 TS28: spleen Present Present 10 µg; total RNA Not Specified Not Specified
Lane 9 P22 TS28: spleen Present Present 10 µg; total RNA Not Specified Not Specified
Lane 10 P32 TS28: spleen Present Present 10 µg; total RNA Not Specified Not Specified
Lane 11 P3 TS27: stomach Present Present 10 µg; total RNA Not Specified Not Specified
Lane 12 P6 TS28: stomach Present Present 10 µg; total RNA Not Specified Not Specified
Lane 13 P10 TS28: stomach Present Present 10 µg; total RNA Not Specified Not Specified
Lane 14 P15 TS28: stomach Present Present 10 µg; total RNA Not Specified Not Specified
Lane 15 P22 TS28: stomach Present Present 10 µg; total RNA Not Specified Not Specified
Lane 16 P32 TS28: stomach Present Present 10 µg; total RNA Not Specified Not Specified
Lane 17 P10 TS28: testis Present Present 10 µg; total RNA Not Specified Male
Lane 18 P15 TS28: testis Present Present 10 µg; total RNA Not Specified Male
Lane 19 P22 TS28: testis Present Present 10 µg; total RNA Not Specified Male
Lane 20 P32 TS28: testis Present Present 10 µg; total RNA Not Specified Male
Lane 21 P3 TS27: thymus Strong Strong 10 µg; total RNA Not Specified Not Specified
Lane 22 P6 TS28: thymus Strong Strong 10 µg; total RNA Not Specified Not Specified
Lane 23 P10 TS28: thymus Strong Strong 10 µg; total RNA Not Specified Not Specified
Lane 24 P15 TS28: thymus Strong Strong 10 µg; total RNA Not Specified Not Specified
Lane 25 P22 TS28: thymus Strong Strong 10 µg; total RNA Not Specified Not Specified
Lane 26 P32 TS28: thymus Strong Strong 10 µg; total RNA Not Specified Not Specified
Notes:
(a) Expression levels in testis increase until P22 then decline. Expression levels in thymus peak at P10-15 then decline. Expression levels in spleen and stomach are either maintained or decline slightly during development.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/06/2026
MGI 6.24 		The Jackson Laboratory