Probe:	NIA clone H3025D11
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Widespread	2_Pfkl E10.5
TS17: brain	Present		2_Pfkl E10.5	This is one of the tissues with the strongest expression level.
TS17: dorsal root ganglion	Present		2_Pfkl E10.5	This is one of the tissues with the strongest expression level.
TS17: eye	Present		2_Pfkl E10.5	This is one of the tissues with the strongest expression level.

Structure	Level	Pattern	Image	Note
TS17: brain	Present		3-PFKLe10.5
TS17: future spinal cord	Present		3-PFKLe10.5
TS17: eye	Strong		3-PFKLe10.5
TS17: otocyst	Absent		3-PFKLe10.5
TS17: dorsal root ganglion	Strong		3-PFKLe10.5
TS17: branchial arch	Present		3-PFKLe10.5
TS17: heart	Present		3-PFKLe10.5
TS17: gut	Present		3-PFKLe10.5
TS17: mesonephros	Present		3-PFKLe10.5
TS17: limb	Present		3-PFKLe10.5
TS17: surface ectoderm	Present		3-PFKLe10.5

Structure	Level	Pattern	Image	Note
TS22: forebrain	Moderate	Regionally restricted	1A12_C58
TS22: midbrain	Absent		1A12_C58
TS22: hindbrain	Moderate	Regionally restricted	1A12_C58
TS22: cerebellum	Absent		1A12_C58
TS22: spinal cord	Moderate	Regionally restricted	1A12_C58
TS22: cranial ganglion	Absent		1A12_C58
TS22: dorsal root ganglion	Weak	Uniform	1A12_C58
TS22: parasympathetic nervous system	Absent		1A12_C58
TS22: eye	Moderate	Regionally restricted	1A12_C58
TS22: olfactory epithelium	Weak	Uniform	1A12_C58
TS22: cochlea	Absent		1A12_C58
TS22: skeleton	Absent		1A12_C58
TS22: lung	Weak	Regionally restricted	1A12_C58
TS22: heart atrium	Moderate	Regionally restricted	1A12_C58
TS22: heart ventricle	Moderate	Regionally restricted	1A12_C58
TS22: arterial system	Absent		1A12_C58
TS22: gut	Moderate	Regionally restricted	1A12_C58
TS22: bladder	Absent		1A12_C58
TS22: metanephros	Weak	Uniform	1A12_C58
TS22: testis	Absent		1A12_C58
TS22: ovary	Absent		1A12_C58
TS22: salivary gland	Moderate	Uniform	1A12_C58
TS22: spleen primordium	Absent		1A12_C58
TS22: pancreas	Weak	Uniform	1A12_C58
TS22: adrenal gland	Absent		1A12_C58
TS22: thyroid gland	Absent		1A12_C58
TS22: thymus primordium	Moderate	Uniform	1A12_C58
TS22: liver	Moderate	Uniform	1A12_C58
TS22: skin	Absent		1A12_C58
TS22: vibrissa epidermal component	Absent		1A12_C58
TS22: skeletal muscle	Absent		1A12_C58
TS22: embryo mesenchyme	Absent		1A12_C58
TS22: tongue	Absent		1A12_C58
TS22: limb	Moderate	Regionally restricted	1A12_C58
TS22: tail	Strong	Regionally restricted	1A12_C58
TS22: choroid plexus	Absent		1A12_C58
TS22: trachea	Weak	Regionally restricted	1A12_C58
TS22: thalamus ventricular layer	Strong		2_Pfkl E14.5	Higher expression is seen in the ventricular zone.
TS22: heart	Present	Uniform	3cc Pfkl E14.5