Gene Expression Data
Assay Details
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Assay
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Reference:
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J:76230
Hajihosseini MK, et al., Expression patterns of fibroblast growth factors-18 and -20 in mouse embryos is suggestive of novel roles in calvarial and limb development. Mech Dev. 2002 Apr;113(1):79-83
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2664829
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Gene symbol:
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Fgf18
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Gene name:
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fibroblast growth factor 18
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Probe:
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Fgf18 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 17.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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C57BL
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B:
embryonic day 17.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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C57BL
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2C:
embryonic day 16.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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C57BL
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2D:
embryonic day 16.5
(more )
Note: Double labeled: purple - Fgf18; orange - Spp1 (osteopontin; assay MGI:2665358).
Specimen
2D:
(close )
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Genetic Background:
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C57BL
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: purple - Fgf18; orange - Spp1 (osteopontin; assay MGI:2665358).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: parietal bone |
Strong |
Regionally restricted |
2D
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Expression is in the dorsal and ventral rims of the parietal bone. Double labeling with Osteopontin indicates that Fgf18 abuts Osteopontin expression but does not colocalize with it and is not in the sutural mesenchymal cells.
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| TS24: frontal bone |
Present |
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2D
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Double labeling with Osteopontin indicates that Fgf18 abuts Osteopontin expression but does not colocalize with it and is not in the sutural mesenchymal cells.
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| TS24: vault of skull |
Present |
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2D
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Double labeling with Osteopontin indicates that Fgf18 abuts Osteopontin expression but does not colocalize with it and is not in the sutural mesenchymal cells.
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Specimen
2E:
embryonic day 16.5
(more )
Note: Double labeled: purple - Fgf18; orange - Spp1 (osteopontin; assay MGI:2665358). Tissue was embedded in 2% agar and a 100-micron-thick vibratome section was cut.
Specimen
2E:
(close )
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Genetic Background:
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C57BL
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Double labeled: purple - Fgf18; orange - Spp1 (osteopontin; assay MGI:2665358). Tissue was embedded in 2% agar and a 100-micron-thick vibratome section was cut.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: vault of skull |
Strong |
Regionally restricted |
2E
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Expression is in the cranial cartilage lying just medial to the parietal bone and posterior to the coronal sutures. Double labeling with Osteopontin indicates that Fgf18 abuts Osteopontin expression but does not colocalize with it.
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Specimen
2F:
embryonic day 16.5
(more )
Note: Double labeled: purple - Fgf18; orange - Spp1 (osteopontin; assay MGI:2665358). Tissue was embedded in 2% agar and a 100-micron-thick vibratome section was cut.
Specimen
2F:
(close )
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Genetic Background:
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C57BL
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Double labeled: purple - Fgf18; orange - Spp1 (osteopontin; assay MGI:2665358). Tissue was embedded in 2% agar and a 100-micron-thick vibratome section was cut.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: vault of skull |
Strong |
Regionally restricted |
2F
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Expression is in the cranial cartilage lying just medial to the parietal bone and posterior to the coronal sutures. Double labeling with Osteopontin indicates that Fgf18 abuts Osteopontin expression but does not colocalize with it.
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Specimen
Not Shown:
embryonic day 17.5
(more )
Note: Tissue was embedded in 2% agar and a 100-micron-thick vibratome section was cut.
Specimen
Not Shown:
(close )
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Genetic Background:
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C57BL
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Tissue was embedded in 2% agar and a 100-micron-thick vibratome section was cut.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: vault of skull |
Present |
Regionally restricted |
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Expression is in the cranial cartilage. Double labeling with Osteopontin indicates that Fgf18 abuts Osteopontin expression but does not colocalize with it and also does not extend to the sutural mesenchymal cells between the two bones.
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Specimen
Not Shown:
embryonic day 9.75
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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C57BL
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: limb |
Absent |
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Specimen
Not Shown:
embryonic day 10.5
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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C57BL
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb |
Absent |
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