Gene Expression Data
Assay Details
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Assay
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Reference:
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J:50852
Chiang PW, et al., Comparison of murine Supt4h and a nearly identical expressed, processed gene: evidence of sequence conservation through gene conversion extending into the untranslated regions. Nucleic Acids Res. 1998 Nov 1;26(21):4960-4
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2657213
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Gene symbol:
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Supt4a
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Gene name:
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SPT4A, DSIF elongation factor subunit
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Probe:
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Supt4-pA, Supt4-pB
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Assay notes:
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Since products from two genes were amplified with this primer set, the PCR products were subjected to diagnostic restriction enzyme digestion. For this assay, BstBI was used. Since both genes were epxressed, only partial digestion was achieved with theenzyme.
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Results
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Image: 3
| Mol. Wt. Marker Lane |
| P adult
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TS28: brain
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Present
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Not Specified; poly-A+ RNA |
Not Specified |
| Not Specified |
| Lane not used in this assay |
| P adult
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TS28: heart
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Present
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Not Specified; poly-A+ RNA |
Not Specified |
| Not Specified |
| Lane not used in this assay |
| P adult
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TS28: liver
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Present
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Not Specified; poly-A+ RNA |
Not Specified |
| Not Specified |
| Lane not used in this assay |
| E17.0
(a)
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TS25: embryo
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Present
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Not Specified; poly-A+ RNA |
Not Specified |
| Not Specified |
| Lane not used in this assay |
| Control |
| Control |
| Control |
| Control |
| Mol. Wt. Marker Lane |
Notes:
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Analysis Tools
