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Gene Expression Data
Assay Details
Assay
Reference: J:40018 Koopman P, et al., Zfy gene expression patterns are not compatible with a primary role in mouse sex determination. Nature. 1989 Dec 21-28;342(6252):940-2
Assay type: RT-PCR
MGI Accession ID: MGI:2655570
Gene symbol: Zfy1
Gene name: zinc finger protein 1, Y-linked
Probe: Zfy1-pC, Zfy1-pD
Probe preparation: RNA
Visualized with: Ethidium bromide
Assay notes: Nested primers were used for this assay. After amplification with the first primer set, the authors used another primer set (MGI:2655583). This gene produces alternative transcripts, as reflected by the three different band sizes.
Results
Image: 2b ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 572 bp 523 bp 477 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
marker Mol. Wt. Marker Lane
9.5 male E9.5 TS15: trunk Absent Absent Absent Not Specified; total RNA Parkes Male embryos posterior to the forelimb bud.
10.5 male E10.5 TS17: trunk Absent Absent Absent Not Specified; total RNA Parkes Male embryos between forelimb and hindlimb buds.
11.5 male E11.5 TS19: gonad Present Present Present Not Specified; total RNA Parkes Male
12.5 male E12.5 TS20: testis Present Present Present Not Specified; total RNA Parkes Male
13.5 male E13.5 TS21: testis Present Present Present Not Specified; total RNA Parkes Male
14.5 male E14.5 TS22: testis Present Present Present Not Specified; total RNA Parkes Male
15.5 male E15.5 TS23: testis Present Present Present Not Specified; total RNA Parkes Male
16.5 male E16.5 TS24: testis Present Present Present Not Specified; total RNA Parkes Male
17.5 male E17.5 TS25: testis Present Present Present Not Specified; total RNA Parkes Male
adult male P adult TS28: testis Present Present Present Not Specified; total RNA Parkes Male
11.5 female E11.5 TS19: gonad Absent Absent Absent Not Specified; total RNA Parkes Female
12.5 female E12.5 TS20: ovary Absent Absent Absent Not Specified; total RNA Parkes Female
13.5 female E13.5 TS21: ovary Absent Absent Absent Not Specified; total RNA Parkes Female
no RNA Control
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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