Zfy1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:40018 Koopman P, et al., Zfy gene expression patterns are not compatible with a primary role in mouse sex determination. Nature. 1989 Dec 21-28;342(6252):940-2
Assay type:	RT-PCR
MGI Accession ID:	MGI:2655566
Gene symbol:	Zfy1
Gene name:	zinc finger protein 1, Y-linked

Probe:	Zfy1-pC, Zfy1-pD
Visualized with:	Ethidium bromide
Assay notes:	Nested primers were used for this assay. After amplification with the first primer set, the authors used another primer set (MGI:2655583). The amplified products also underwent Southern blotting, as pictured in Fig. 2a2.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Testis 0.1	P adult	TS28: testis	Present	0.1 µg; total RNA	Parkes		Male
Testis 0.01	P adult	TS28: testis	Present	0.01 µg; total RNA	Parkes		Male
Ov.	P adult	TS28: ovary	Absent	1 µg; total RNA	Parkes		Female
Li.	P adult	TS28: liver	Absent	1 µg; total RNA	Parkes		Male
9.5 male	E9.5	TS15: trunk	Absent	1 µg; total RNA	Parkes		Male	embryos posterior to the forelimb bud
9.5 female	E9.5	TS15: trunk	Absent	1 µg; total RNA	Parkes		Female	embryos posterior to the forelimb bud
10.5 male	E10.5	TS17: trunk	Present	1 µg; total RNA	Parkes		Male	embryos between forelimb and hindlimb buds
10.5 female	E10.5	TS17: trunk	Absent	1 µg; total RNA	Parkes		Female	embryos between forelimb and hindlimb buds
11.5 male	E11.5	TS19: gonad	Present	1 µg; total RNA	Parkes		Male
11.5 female	E11.5	TS19: gonad	Absent	1 µg; total RNA	Parkes		Female
12.5 male	E12.5	TS20: testis	Present	1 µg; total RNA	Parkes		Male
12.5 female	E12.5	TS20: ovary	Absent (a)	1 µg; total RNA	Parkes		Female
13.5 male	E13.5	TS21: testis	Present	1 µg; total RNA	Parkes		Male
13.5 female	E13.5	TS21: ovary	Absent	1 µg; total RNA	Parkes		Female
14.5 male	E14.5	TS22: testis	Present	1 µg; total RNA	Parkes		Male
14.5 female	E14.5	TS22: ovary	Absent	1 µg; total RNA	Parkes		Female
ES male	In vitro data: no data stored
ES female	In vitro data: no data stored
No RNA	Control
11.5 male He	E11.5	TS19: head	Absent	1 µg; total RNA	Parkes		Male

Notes:
(a) There is a minor signal in this lane, but the authors believe that it is due to incorrectly sexed specimens, rather than expression in the female.

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