Il11ra2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:33356 Robb L, et al., Structural analysis of the gene encoding the murine interleukin-11 receptor alpha-chain and a related locus. J Biol Chem. 1996 Jun 7;271(23):13754-61
Assay type:	RT-PCR
MGI Accession ID:	MGI:2655362
Gene symbol:	Il11ra2
Gene name:	interleukin 11 receptor subunit alpha 2

Probe:

Il11ra-pA, Il11ra-pB

Assay notes:

The purpose of this assay was to differentiate between Il11ra1 and Il11ra2 in the tissue by the differential pattern of restriction enzyme digestion. In this amplified fragment, Il11ra2 has a BamHI site and Il11ra1 does not. The amplified products were undigested 170bp bands (-) or digested with BamHI (+), Southern blotted and detected with a mixture of 2 probes. The probes were designed such that all restriction fragments would be hybridized.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	170 bp (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
1-	Control
1+	P	TS28: bone marrow	Absent	0.05 µg; total RNA	BALB/cBy		Not Specified
2-	Control
2+	P	TS28: bone marrow	Absent	0.05 µg; total RNA	C3H/HeJ		Not Specified
3-	Control
3+	P	TS28: bone marrow	Absent	0.05 µg; total RNA	C57BL/6J		Not Specified
4-	Control
4+	P	TS28: bone marrow	Absent	0.05 µg; total RNA	129X1/SvJ		Not Specified
5-	Control
5+	P	TS28: bone marrow	Absent	0.05 µg; total RNA	DBA/2J		Not Specified
6-	Control
6+	P	TS28: bone marrow	Absent	0.05 µg; total RNA	SWR/J		Not Specified
7-	Control
7+	P	TS28: bone marrow	Absent	0.05 µg; total RNA	SJL/J		Not Specified

Notes:
(a) The absence of any BamHI digested fragments indicates that no Il11ra2 was present in this tissue.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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