Gene Expression Data
Assay Details
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Assay
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Reference:
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J:36725
Kim KK, et al., Mouse RAD50 has limited epitopic homology to p53 and is expressed in the adult myocardium. J Biol Chem. 1996 Nov 15;271(46):29255-64
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Assay type:
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Northern blot
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MGI Accession ID:
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MGI:2655075
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Gene symbol:
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Rad50
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Gene name:
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RAD50 double strand break repair protein
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Probe:
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Rad50 cDNA1
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Probe preparation:
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Double stranded labelled with radioactivity DNA
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Visualized with:
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Autoradiography
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Assay notes:
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The probe was radiolabeled. Densitometry was used to determine the relative level (%) of the 5.3kb transcript in each tissue as compared to AT-2 cardiomyocytes on the same blot. The values were further normalized to controls to account for variations inloading.
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Results
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| In vitro data: no data stored |
| P1.5
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TS27: brain
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Weak
(a)
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Not Specified
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10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
| P1.5
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TS27: gut
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Weak
(a)
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Not Specified
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10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
| P1.5
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TS27: heart
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Strong
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Present
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10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
| P1.5
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TS27: metanephros
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Not Specified
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Not Specified
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10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
| P1.5
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TS27: liver
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Weak
(a)
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Not Specified
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10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
| P1.5
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TS27: lung
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Present
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Present
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10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
| P1.5
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TS27: skeletal muscle
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Not Specified
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Not Specified
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10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
| P1.5
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TS27: skin
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Weak
(a)
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Not Specified
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10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
| P1.5
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TS27: spleen
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Present
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Not Specified
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10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
| P1.5
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TS27: thymus
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Not Specified
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Not Specified
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10 µg; total RNA |
C3HeB/FeJ |
| Not Specified |
Notes:
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Analysis Tools