About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:36725 Kim KK, et al., Mouse RAD50 has limited epitopic homology to p53 and is expressed in the adult myocardium. J Biol Chem. 1996 Nov 15;271(46):29255-64
Assay type: Northern blot
MGI Accession ID: MGI:2655073
Gene symbol: Rad50
Gene name: RAD50 double strand break repair protein
Probe: Rad50 cDNA1
Probe preparation: Double stranded labelled with radioactivity DNA
Visualized with: Autoradiography
Assay notes: The probe was radiolabeled. Densitometry was used to determine the relative level (%) of the transcript in each tissue as compared to AT-2 cardiomyocytes on the same blot. The values were further normalized to controls to account for variations in loading.
Results
Image: 6A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 5.3 kb (a) Amount Genetic BackgroundMutant Allele(s)Sex
AT-2 (100%) In vitro data: no data stored
BRAIN (38%) E18.0 (b) TS26: brain Present 10 µg; total RNA C3HeB/FeJ Not Specified
GUT (36%) E18.0 (b) TS26: gut Present 10 µg; total RNA C3HeB/FeJ Not Specified
HEART (60%) E18.0 (b) TS26: heart Strong 10 µg; total RNA C3HeB/FeJ Not Specified
KIDNEY (44%) E18.0 (b) TS26: metanephros Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
LIVER (57%) E18.0 (b) TS26: liver Strong 10 µg; total RNA C3HeB/FeJ Not Specified
LUNG (25%) E18.0 (b) TS26: lung Present 10 µg; total RNA C3HeB/FeJ Not Specified
SK. MUS. (5%) E18.0 (b) TS26: skeletal muscle Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
SKIN (14%) E18.0 (b) TS26: skin Present 10 µg; total RNA C3HeB/FeJ Not Specified
SPLEEN (15%) E18.0 (b) TS26: spleen Not Specified 10 µg; total RNA C3HeB/FeJ Not Specified
THY (57%) E18.0 (b) TS26: thymus Strong 10 µg; total RNA C3HeB/FeJ Not Specified
Notes:
(a) This transcript was present in most tissues at this stage.
(b) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/21/2024
MGI 6.23 		The Jackson Laboratory