Pxn Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:49586 Mazaki Y, et al., Paxillin isoforms in mouse. Lack of the gamma isoform and developmentally specific beta isoform expression. J Biol Chem. 1998 Aug 28;273(35):22435-41
Assay type:	Western blot
MGI Accession ID:	MGI:2653445
Gene symbol:	Pxn
Gene name:	paxillin

Antibody:	Ab 199-217
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	The protein for this assay was obtained by immunoprecipitation using the Pxn monoclonal antibody MGI:2653339, which recognizes all isoforms. Authors state that smearing of bands is caused by the tyrosine phosphorylation of a fraction of Pxn in embryonic tissues. The antibody used in this assay recognizes the alpha and beta isoforms.

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Size Not Specified (b)	Genetic Background	Mutant Allele(s)	Sex
GST-pax alpha	In vitro data: no data stored
GST-pax beta	In vitro data: no data stored
9.5 day	E9.5 (c)	TS15: embryo	Present (d)	Present	BALB/c		Not Specified
11.5 day	E11.5 (c)	TS19: embryo	Present (d)	Present	BALB/c		Not Specified
15.5 day	E15.5 (c)	TS23: embryo	Present (e)	Present	BALB/c		Not Specified
17.5 day	E17.5 (c)	TS25: embryo	Present (e)	Present	BALB/c		Not Specified
17.5 day (Mouse IgG1)	Control

Notes:
(a) Upper band is the beta isoform.
(b) Lower band is the alpha isoform. The level of expression of this isoform remained constant through the embryonic stages examined.
(c) Age of embryo at noon of plug day not specified in reference.
(d) The beta isoform level of expression was 2-3 times lower than that of the alpha isoform.
(e) The beta isoform level of expression was equal to that of the alpha isoform.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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