Cgn Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:81303 Javed Q, et al., Tight junction protein cingulin is expressed by maternal and embryonic genomes during early mouse development. Development. 1993 Mar;117(3):1145-51
Assay type:	Western blot
MGI Accession ID:	MGI:2653421
Gene symbol:	Cgn
Gene name:	cingulin

Antibody:	anti-Cingulin
Detection system:	Secondary antibody coupled to I125

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Genetic Background	Mutant Allele(s)	Sex	Note
M	Mol. Wt. Marker Lane
L	P	TS28: liver	Present	MF1		Not Specified
E	P	TS28: secondary oocyte	Strong	MF1		Female
2/4	E1.5-2.0 (b)	TS2: 2-cell stage conceptus TS3: 4-cell stage conceptus	Strong	MF1		Not Specified	Late 2 cell/early 4 cell embryos.
E8	E2.5 (b)	TS3: 8-cell stage conceptus	Moderate	MF1		Not Specified	Precompact 8 cell embryos.
C8	E3.0 (b)	TS3: morula-stage conceptus	Moderate	MF1		Not Specified	Compact 8 cell embryos.
EB	E3.5 (b)	TS4: blastocyst-stage conceptus	Moderate	MF1		Not Specified	Early blastocyst.
EB	E3.5 (b)	TS4: blastocyst-stage conceptus	Moderate	MF1		Not Specified	Early blastocyst.
LB	E5.0 (b)	TS5: blastocyst-stage conceptus	Very strong	MF1		Not Specified	Late blastocyst.
M	Mol. Wt. Marker Lane

Notes:
(a) There were two bands present in liver, one in embryos. Strengths of staining were assigned by a curator based on the densitometry data reported in Fig. 1B.
(b) Age assigned by curator using morphological criteria supplied by author.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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