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Gene Expression Data
Assay Details
Assay
Reference: J:31483 Park CH, et al., Expression of multiple forms of 3 beta-hydroxysteroid dehydrogenase in the mouse liver during fetal and postnatal development. Mol Cell Endocrinol. 1996 Feb 5;116(2):157-64
Assay type: RT-PCR
MGI Accession ID: MGI:2451013
Gene symbol: Hsd3b2
Gene name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Probe: primer pair 1
Visualized with: Ethidium bromide
Assay notes: The primers used were designed to amplify all family members. (This amplicon is present at 629 bp.) Individual family members were identified by subjecting the amplified fragment to restriction enzyme digestion. Each analysis represents results from at least three separate tissue samples at each age.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 629 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex
13.5 U Control
13.5 D E13.5 TS21: liver Absent .2 µg; total RNA C57BL/6J Male
15.5 U Control
15.5 D E15.5 TS23: liver Absent .2 µg; total RNA C57BL/6J Male
17.5 U Control
17.5 D E17.5 TS25: liver Weak .2 µg; total RNA C57BL/6J Male
1 U Control
1 D P1 TS27: liver Weak .2 µg; total RNA C57BL/6J Male
7 U Control
7 D P7 TS28: liver Weak .2 µg; total RNA C57BL/6J Male
10 U Control
10 D P10 TS28: liver Weak .2 µg; total RNA C57BL/6J Male
16 U Control
16 D P16 TS28: liver Weak .2 µg; total RNA C57BL/6J Male
20 U Control
20 D P20 TS28: liver Weak .2 µg; total RNA C57BL/6J Male
30 U Control
30 D P30 TS28: liver Weak .2 µg; total RNA C57BL/6J Male
40 U Control
40 D P40 TS28: liver Weak .2 µg; total RNA C57BL/6J Male
50 U Control
50 D P50 TS28: liver Weak .2 µg; total RNA C57BL/6J Male
Notes:
(a) Levels of expression are higher in male livers compared to female livers.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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