Gene Expression Data
Assay Details
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Assay
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Reference:
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J:51129
Bierkamp C, et al., Desmosomal localization of beta-catenin in the skin of plakoglobin null-mutant mice. Development. 1999 Jan;126(2):371-81
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2449081
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Gene symbol:
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Ctnnb1
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Gene name:
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catenin beta 1
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Antibody:
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anti-Beta-catenin (C2206)
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Detection system:
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Secondary antibody coupled to colloidal gold
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Assay notes:
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The tissue was fixed in 3% paraformaldehyde for 10 min., followed by 8% paraformaldehyde overnight.
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Results
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Specimen
5E:
embryonic day 17.5
(more  )
Note: Embedded in methacrylate.
Specimen
5E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: gut |
Present |
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5E
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Expression was detected in the adherens junctions but was absent from the desmosomes of the intestinal epithelium.
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Specimen
5F:
embryonic day 17.5; Juptm1Kem/ Juptm1Kem
(more )
Note: Embedded in methacrylate.
Specimen
5F:
(close )
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Genetic Background:
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either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
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Mutant Allele(s):
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Juptm1Kem/Juptm1Kem
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: gut |
Present |
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5F
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Expression was detected in the adherens junctions and the desmosomes of the intestinal epithelium.
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Specimen
5I:
embryonic day 17.5
(more )
Note: Embedded in methacrylate.
Specimen
5I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
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5I
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Expression was detected in the adherens junctions but was absent from the desmosomes of the basal and spinous cell layers of facial skin.
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Specimen
5K:
embryonic day 17.5; Juptm1Kem/ Juptm1Kem
(more )
Note: Embedded in methacrylate.
Specimen
5K:
(close )
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Genetic Background:
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either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
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Mutant Allele(s):
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Juptm1Kem/Juptm1Kem
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
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5K
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Expression was detected in the adherens junctions and the desmosomes of the basal and spinous cell layers of facial skin.
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Analysis Tools