Gene Expression Data
Assay Details
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Assay
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Reference:
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J:51129
Bierkamp C, et al., Desmosomal localization of beta-catenin in the skin of plakoglobin null-mutant mice. Development. 1999 Jan;126(2):371-81
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2449076
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Gene symbol:
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Ctnnb1
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Gene name:
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catenin beta 1
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Antibody:
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anti-Beta-catenin (C2206)
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Detection system:
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Secondary antibody coupled to Cy3
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Assay notes:
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The tissue was fixed in 3% paraformaldehyde for 10 min., followed by 8% paraformaldehyde overnight.
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Results
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Specimen
2G:
embryonic day 17.5
(more  )
Note: Embedded in methacrylate.
Specimen
2G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
Graded |
2G
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Expression was strong in the basal cell layer and reduced or absent in the granular layer and stratum corneum. Expression was detected in the spinous cell layer and adherens junctions.
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Specimen
2H:
embryonic day 17.5; Juptm1Kem/ Juptm1Kem
(more )
Note: Embedded in methacrylate.
Specimen
2H:
(close )
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Genetic Background:
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either: (involves: 129 * 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)
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Mutant Allele(s):
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Juptm1Kem/Juptm1Kem
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
Graded |
2H
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Expression was strong in the basal cell layer and reduced or absent in the granular layer and stratum corneum. Expression was detected in the spinous cell layer and adherens junctions. No differences were observed between mutant and wild type staining.
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Analysis Tools