Probe:	Tropoelastin probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The molecular probe (MGI:2448750) may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Present	Regionally restricted	3A	Expression was detected adjacent to the proximal epithelial tubules but absent from the mesenchyme surrounding the epithelial buds.

Structure	Level	Pattern	Image	Note
TS23: lung mesenchyme	Present	Regionally restricted	3B	Expression was detected adjacent to the proximal epithelial tubules but absent from the mesenchyme surrounding the epithelial buds. Expression in mutant was the same as in wild type.

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Present	Regionally restricted	3C	Expression was detected adjacent to the proximal epithelial tubules but absent from the mesenchyme surrounding the epithelial buds.

Structure	Level	Pattern	Image	Note
TS26: lung mesenchyme	Strong	Regionally restricted	3D	Expression was detected in all mesenchymal cells at both proximal and distal sites.
TS26: lung vascular element	Strong	Regionally restricted	3D	Expression was detected in the endothelium and the smooth muscle cells of the walls.

Structure	Level	Pattern	Image	Note
TS26: lung mesenchyme	Strong	Regionally restricted	3E	Expression was detected in all mesenchymal cells at both proximal and distal sites.
TS26: lung vascular element	Strong	Regionally restricted	3E	Expression was detected in the endothelium and the smooth muscle cells of the walls.

Structure	Level	Pattern	Image	Note
TS26: lung mesenchyme	Strong	Regionally restricted	3F	Expression was detected in all mesenchymal cells at both proximal and distal sites. Expression in mutant was the same as in wild type.
TS26: lung vascular element	Strong	Regionally restricted	3F	Expression was detected in the endothelium and the smooth muscle cells of the walls. Expression in mutant was the same as in wild type.

Structure	Level	Pattern	Image	Note
TS27: lung vascular element	Strong		3G
TS27: lung connective tissue	Weak	Regionally restricted	3G	Expression was weak in most mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS27: lung vascular element	Strong		3H
TS27: lung connective tissue	Weak	Regionally restricted	3H	Expression was weak in most mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS27: lung vascular element	Strong		3I
TS27: lung connective tissue	Weak	Regionally restricted	3I	Expression was weak in most mesenchymal cells. Expression in mutant was the same as in wild type.

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present		3J
TS28: bronchus	Present	Regionally restricted	3J	Expression in the bronchial walls.
TS28: lung	Present	Regionally restricted	3J	Expression in scattered mesenchymal cells, usually found at ingrowing alveolar septa.

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present		3K
TS28: bronchus	Present	Regionally restricted	3K	Expression in the bronchial walls.
TS28: lung connective tissue	Present	Regionally restricted	3K	Expression in scattered mesenchymal cells, usually found at ingrowing alveolar septa.

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present		3L
TS28: bronchus	Present	Regionally restricted	3L	Expression in bronchial walls.
TS28: lung connective tissue	Absent		3L	Expression was absent from mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present		3M
TS28: bronchus	Present	Regionally restricted	3M	Expression in bronchial walls.
TS28: lung connective tissue	Absent		3M	Expression was absent from mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present
TS28: bronchus	Present	Regionally restricted		Expression in bronchial walls.
TS28: lung connective tissue	Present	Regionally restricted		Expression in scattered mesenchymal cells, usually found at ingrowing alveolar septa.

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Present
TS28: bronchus	Present	Regionally restricted		Expression in bronchial walls.
TS28: lung connective tissue	Absent			Expression was absent from mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS28: alveolar septum	Present	Regionally restricted	4A	Expression was detected at the alveolar septa and the mesenchymal cells.
TS28: lung connective tissue	Present	Regionally restricted	4A	Expression was detected at the alveolar septa and the mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS28: alveolar septum	Present	Regionally restricted	4B	Expression was detected at the alveolar septa and the mesenchymal cells.
TS28: lung connective tissue	Present	Regionally restricted	4B	Expression was detected at the alveolar septa and the mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS28: alveolus smooth muscle	Absent			Expression was absent from the alveolar smooth muscle cells.