Gene Expression Data
Assay Details
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Assay
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Reference:
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J:78334
Liu W, et al., Sonic hedgehog regulates otic capsule chondrogenesis and inner ear development in the mouse embryo. Dev Biol. 2002 Aug 15;248(2):240-50
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2447389
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Antibody:
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Anti-Shh
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The fixative was methacarn.
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Results
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Specimen
1A:
embryonic day 10.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Graded |
1A
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Expression was detected in the epithelium with greatest intensity along its lateral wall.
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| TS17: inner ear |
Present |
Regionally restricted |
1A
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Expression was detected in the cells bordering the statoacoustic ganglion complex and the lateral periotic mesenchyme.
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Specimen
1C:
embryonic day 12.0
(more )
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: inner ear |
Present |
Regionally restricted |
1C
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Expression was detected in the otocyst epithelium and the condensing periotic mesenchyme surrounding the lateral wall.
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| TS20: cochlear duct |
Strong |
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1C
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Expression was detected in the ventromedial aspect.
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Specimen
1D:
embryonic day 14.0
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
1E:
embryonic day 14.0
(more )
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
1F:
embryonic day 14.0
(more )
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: inner ear |
Present |
Regionally restricted |
1F
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Expression was detected in the periotic mesenchyme surrounding the semicircular ducts.
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Analysis Tools