Gene Expression Data
Assay Details
Assay
|
Reference:
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J:58627
Grotewold L, et al., Expression pattern of Dkk-1 during mouse limb development. Mech Dev. 1999 Dec;89(1-2):151-3
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Assay type:
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RNA in situ
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MGI Accession ID:
|
MGI:2388203
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with fluorescein RNA
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Visualized with:
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Alkaline phosphatase
|
|
Results
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Specimen
2A,B:
embryonic day 10.5
(more )
Specimen
2A,B:
(close )
Genetic Background:
|
involves: C3H * C57BL/6
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Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindlimb bud mesenchyme |
Present |
Regionally restricted |
2B
,
2A
|
Expression is in the zone of polarizing activity. The expression domain is distinct from that of Dkk1 (see assay MGI:1329040).
|
Specimen
2C:
embryonic day 10.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
involves: C3H * C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
2C
|
Expression is in the zone of polarizing activity. The expression domain is distinct from that of Dkk1 (see assay MGI:1329040).
|
Specimen
Not Shown:
embryonic day 11.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
|
involves: C3H * C57BL/6
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: limb mesenchyme |
Present |
Regionally restricted |
|
Expression is in the zone of polarizing activity. The expression domain is distinct from that of Dkk1 (see assay MGI:1329040).
|
|