Gene Expression Data
Assay Details
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Assay
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Reference:
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J:69842
Lustig M, et al., Nr-CAM expression in the developing mouse nervous system: ventral midline structures, specific fiber tracts, and neuropilar regions. J Comp Neurol. 2001 May 21;434(1):13-28
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2387141
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Gene symbol:
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Nrcam
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Gene name:
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neuronal cell adhesion molecule
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Antibody:
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anti-Nr-CAM
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Detection system:
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Secondary antibody
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Results
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Specimen
4A:
embryonic day 14.5
(more  )
Note: This is a frontal section through the diencephalon.
Specimen
4A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is a frontal section through the diencephalon.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4A
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Staining is present in midline glial cells in the ventral region and on either side of the midline, as well as in radial glia.
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| TS22: optic chiasma |
Present |
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4A
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Staining is present in the optic fibers coursing through the midline.
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Specimen
4C:
embryonic day 14.5
(more )
Note: This is a horizontal section through the diencephalon.
Specimen
4C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is a horizontal section through the diencephalon.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4C
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Staining is present in midline glial cells in the ventral region and on either side of the midline, as well as in radial glia.
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| TS22: optic chiasma |
Present |
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4C
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Specimen
4E:
embryonic day 14.5
(more )
Note: This is an (anterior) frontal section through the diencephalon; also stained for Cntn2 (Tag1; assay MGI:2387142).
Specimen
4E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is an (anterior) frontal section through the diencephalon; also stained for Cntn2 (Tag1; assay MGI:2387142).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic nerve |
Present |
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4E
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Staining is present in the optic nerve and tract of the retinal axons at the midline of the diencephalon.
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| TS22: optic tract |
Present |
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4E
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Staining is present in the optic nerve and tract of the retinal axons at the midline of the diencephalon.
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Specimen
4H:
embryonic day 14.5
(more )
Note: This is a (posterior) frontal section through the diencephalon; also stained for Cntn2 (Tag1; assay MGI:2387142).
Specimen
4H:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is a (posterior) frontal section through the diencephalon; also stained for Cntn2 (Tag1; assay MGI:2387142).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic nerve |
Present |
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4H
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Staining is present in the optic nerve and tract of the retinal axons at the midline of the diencephalon.
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| TS22: optic tract |
Present |
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4H
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Staining is present in the optic nerve and tract of the retinal axons at the midline of the diencephalon.
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Specimen
4K:
embryonic day 14.5
(more )
Note: This is a (dorsal) horizontal section through the diencephalon; also stained for Cntn2 (Tag1; assay MGI:2387142).
Specimen
4K:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
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|
Note:
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This is a (dorsal) horizontal section through the diencephalon; also stained for Cntn2 (Tag1; assay MGI:2387142).
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Specimen
4N:
embryonic day 14.5
(more )
Note: This is a (ventral) horizontal section through the diencephalon; also stained for Cntn2 (Tag1; assay MGI:2387142).
Specimen
4N:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
This is a (ventral) horizontal section through the diencephalon; also stained for Cntn2 (Tag1; assay MGI:2387142).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic nerve |
Present |
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4N
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Staining is present in the retinal axons at the midline of the diencephalon.
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Specimen
5A:
embryonic day 16.5
(more )
Note: This is a horizontal section through the retina; also stained for Cntn2 (Tag1; assay MGI:2387142).
Specimen
5A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
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This is a horizontal section through the retina; also stained for Cntn2 (Tag1; assay MGI:2387142).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: neural retina |
Present |
Regionally restricted |
5A
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Staining is present throughout the retinal layers, including the retinal and inner plexiform layers. There is weak staining in the cell bodies and axons of the retinal ganglion cells.
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| TS24: optic tract |
Weak |
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5A
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Staining is present in the retinal axons in the optic tract.
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Specimen
5D:
embryonic day 16.5
(more )
Note: This is a frontal section through the optic chiasm region; also stained for Cntn2 (Tag1; assay MGI:2387142).
Specimen
5D:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
This is a frontal section through the optic chiasm region; also stained for Cntn2 (Tag1; assay MGI:2387142).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: diencephalon |
Present |
Regionally restricted |
5D
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Staining is present in the vertically oriented radial glial fibers and in the cells bodies just below the ventricle.
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| TS24: optic chiasma |
Present |
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5D
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Staining is present in the retinal axons coursing through the midline.
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Specimen
5G:
embryonic day 16.5
(more )
Note: This is a horizontal section through the optic chiasm region; also stained for Cntn2 (Tag1; assay MGI:2387142).
Specimen
5G:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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This is a horizontal section through the optic chiasm region; also stained for Cntn2 (Tag1; assay MGI:2387142).
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