Probe:	Opr(3')
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present	Uniform	2A

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present		2B
TS10: embryo ectoderm	Present	Graded	2B	Expression is stronger in the anterior half of the ectoderm.

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present		2C
TS10: embryo ectoderm	Present		2C	Expression is stronger in the anterior half of the ectoderm.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Graded	2D	Expression is intense at the anterior.

Structure	Level	Pattern	Image	Note
TS11: future spinal cord neural plate	Present	Regionally restricted	2E	There is expression everywhere but the midline.

Structure	Level	Pattern	Image	Note
TS11: future spinal cord neural plate	Present	Regionally restricted	2F	There is expression everywhere but the midline.

Structure	Level	Pattern	Image	Note
TS12: neural tube	Present	Regionally restricted	2G	Expression is at the dorsal side of the neural tube.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	2H	Expression is on the dorsal side of the closing neural plate.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	2I	Expression is on the dorsal side of the closing neural plate.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	2J	Expression is on the dorsal side of the closing neural plate.

Structure	Level	Pattern	Image	Note
TS13: trunk mesenchyme derived from neural crest	Absent		2K
TS13: trunk somite	Absent		2K

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	2L	Expression is in the dorsal midline.
TS14: eye	Present		2L
TS14: neural tube roof plate	Present		2L
TS14: head somite	Absent		2L
TS14: trunk unsegmented mesenchyme	Present	Uniform	2O , 2L
TS14: trunk somite	Present	Regionally restricted	2O , 2L	Expression is at the dorsal-most side of each somite.
TS14: limb	Present		2L

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present	Regionally restricted	2M	Expression is along the dorsomedial edge of the cerebral hemispheres. Expression is in the lamina terminalis but not in the choroidal roof.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	2N	Expression is dorsal.
TS17: tail neural tube	Present	Regionally restricted	2N	Expression is dorsal.
TS17: neural tube roof plate	Present		2N

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3A	The expression domain is divided into anterior and posterior mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: future arm mesenchyme	Present	Regionally restricted	3B	Expression is in the wrist.
TS19: handplate mesenchyme	Present	Regionally restricted	3B	Expression is in the wrist.

Structure	Level	Pattern	Image	Note
TS20: forearm mesenchyme	Present	Regionally restricted	3C	Expression is in the wrist.
TS20: forelimb interdigital region mesenchyme	Present		3C

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	3D	Expression is adjacent to the apical ectodermal irdge.

Structure	Level	Pattern	Image	Note
TS19: footplate mesenchyme	Present	Regionally restricted	3E	The expression domain is divided into anterior and posterior mesenchyme.
TS19: future leg mesenchyme	Present	Regionally restricted	3E	The expression domain is divided into anterior and posterior mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: hindlimb interdigital region mesenchyme	Present		3F
TS20: lower leg mesenchyme	Present		3F