Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63215
Schwitzgebel VM, et al., Expression of neurogenin3 reveals an islet cell precursor population in the pancreas. Development. 2000 Aug;127(16):3533-42
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2386020
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Gene symbol:
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Ascl1
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Gene name:
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achaete-scute family bHLH transcription factor 1
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Probe:
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clone 2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1C:
embryonic day 15.5
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas |
Present |
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1C
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Authors report low expression in the pancreas.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pancreas |
Absent |
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Authors report no expression in the pancreas at this stage.
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| TS20: enteric nervous system |
Present |
Single cells |
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Authors report expression in scattered cells surrounding the pancreas, probably due to expression in developing enteric neurons.
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Analysis Tools
