Gene Expression Data
Assay Details
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Assay
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Reference:
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J:78220
Zhang Z, et al., Rescue of cleft palate in Msx1-deficient mice by transgenic Bmp4 reveals a network of BMP and Shh signaling in the regulation of mammalian palatogenesis. Development. 2002 Sep;129(17):4135-46
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2383971
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Gene symbol:
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Bmp4
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Gene name:
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bone morphogenetic protein 4
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Probe:
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Bmp4 cDNA5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2D:
embryonic day 12.5
(more  )
Specimen
2D:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2E:
embryonic day 13.5
(more )
Specimen
2E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf mesenchyme |
Present |
Regionally restricted |
2E
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Expression in anterior palatal shelf, restricted to mesenchyme adjacent to the medial edge epithelium.
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Specimen
2F:
embryonic day 13.5
(more )
Specimen
2F:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf mesenchyme |
Present |
Regionally restricted |
2F
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There is no expression in posterior region of palatal shelf, although expression is present elsewhere.
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Specimen
3A:
(close )
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Genetic Background:
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involves: 129S4/SvJae * CD-1
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: palatal shelf epithelium |
Present |
Regionally restricted |
3A
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Authors state that expression is downregulated in mutant palatal mesenchyme, but remained unaltered in epithelium.
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| TS20: palatal shelf mesenchyme |
Present |
Regionally restricted |
3A
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Authors state that expression is downregulated in mutant palatal mesenchyme, but remained unaltered in epithelium.
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Specimen
3B:
(close )
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Genetic Background:
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involves: 129S4/SvJae * CD-1
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Mutant Allele(s):
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Msx1tm1Rilm/Msx1tm1Rilm
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools