Antibody:	Eps15R antibody
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Present	Regionally restricted	1B	Staining in post-mitotic neurons.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex ventricular layer	Present	Regionally restricted	1B inset	Nuclear staining.

Structure	Level	Pattern	Image	Note
TS20: spinal ganglion	Ambiguous	Regionally restricted	1G	Staining is unclear, with clear sparing of axons.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Weak	Regionally restricted	1H	Expression in motor neurons.

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present		1I	Expression is restricted to the ganglion cell layer and appears nuclear.

Structure	Level	Pattern	Image	Note
TS20: diencephalon floor plate	Present
TS20: remnant of Rathke's pouch	Present

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present
TS22: nose	Present	Regionally restricted		nasal epithelium.
TS22: ear	Present	Regionally restricted		auditory vesicle.

Structure	Level	Pattern	Image	Note
TS22: collecting duct	Present	Regionally restricted	3D	Staining in all epithelia.
TS22: maturing glomerular tuft	Present	Regionally restricted	3D	Staining in all epithelia.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present		3E

Structure	Level	Pattern	Image	Note
TS22: gut	Present	Regionally restricted	3F	Expression in epitleia, smooth muscle, and mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Regionally restricted		Present in post-mitotic neurons. Absent in germinal layer.
TS20: choroid plexus	Present
TS20: cranial ganglion	Present
TS20: nose	Present			nasal epithelium.
TS20: ear	Present			auditory vesicle.
TS20: retina	Present
TS20: spinal ganglion	Present
TS20: nerve	Absent			nerve tracts.
TS20: heart	Weak
TS20: liver	Weak
TS20: lung	Weak
TS20: metanephros	Weak
TS20: gut	Present			intestine.
TS20: pancreas	Strong
TS20: remnant of Rathke's pouch	Present
TS20: skeleton	Weak			bones and cartilage.
TS20: integumental system	Absent			skin.

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Present	Regionally restricted		Present in post-mitotic neurons. Absent in germinal layer.
TS22: choroid plexus	Weak
TS22: cranial ganglion	Weak
TS22: nose	Weak			nasal epithelium.
TS22: ear	Weak			auditory vesicle.
TS22: retina	Weak			auditory vesicle.
TS22: spinal ganglion	Weak			auditory vesicle.
TS22: nerve	Absent			nerve tracts.
TS22: spinal cord	Weak			motor neurons.
TS22: integumental system	Absent			Absent in whisker pad and in skin.
TS22: heart	Weak
TS22: outflow tract aortic component	Absent
TS22: liver	Weak
TS22: lung	Weak
TS22: metanephros	Weak
TS22: esophagus	Absent
TS22: gut	Weak			intestine.
TS22: pancreas	Present
TS22: remnant of Rathke's pouch	Present
TS22: salivary gland	Present
TS22: skeleton	Weak			bones and cartilage.