Gene Expression Data
Assay Details
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Assay
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Reference:
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J:77682
Zhu J, et al., beta8 integrins are required for vascular morphogenesis in mouse embryos. Development. 2002 Jun;129(12):2891-903
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2182611
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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MEC 13.3
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Detection system:
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Secondary antibody
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Results
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Specimen
not shown:
(close  )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6J)
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Mutant Allele(s):
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Itgb8tm1Lfr/Itgb8tm1Lfr
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Carnoy's Fixative
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Specimen
not shown:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6J)
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Mutant Allele(s):
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Itgb8tm1Lfr/Itgb8tm1Lfr
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Carnoy's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: cardiovascular system |
Present |
Regionally restricted |
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Authors report that major blood vessels, such as dorsal aorta, cardinal veins, intersomitic arteries and intracerebral arteries are present in mutant embryos.
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Specimen
6E1, 6E3:
embryonic day 12.5
(more  )
Specimen
6E1, 6E3:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: C57BL/6J)
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Specimen
6F1, 6F3:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6J)
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Mutant Allele(s):
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Itgb8tm1Lfr/Itgb8tm1Lfr
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cardiovascular system |
Present |
Regionally restricted |
6F
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Authors report brain capillary vessels exhibit irregular distended morphologies and are often conjoined.
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Specimen
8A:
embryonic day 10.5
(more )
Specimen
8A:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: C57BL/6J)
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: cardiovascular system |
Present |
Regionally restricted |
8A
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Capillary vessels in the brain grow close to and couple near the ventricle.
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Specimen
8B:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6J)
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Mutant Allele(s):
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Itgb8tm1Lfr/Itgb8tm1Lfr
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: cardiovascular system |
Present |
Regionally restricted |
8B
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Brain capillary vessels extend a shorter distance into the neuroepithelium and couple further away from the ventricle.
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Specimen
8C:
embryonic day 12.5
(more )
Specimen
8C:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: C57BL/6J)
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cardiovascular system |
Present |
Regionally restricted |
8C
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Brain capillary vessels have branched and anastomosed to form an organized network.
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Specimen
8D:
(close )
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Genetic Background:
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either: (involves: 129/Sv) or (involves: 129/Sv * C57BL/6J)
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Mutant Allele(s):
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Itgb8tm1Lfr/Itgb8tm1Lfr
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cardiovascular system |
Present |
Regionally restricted |
8D
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Brain capillary vessels appear bulbous, tortuous and irregular; they have not invaded regions of neuroepithelium immediately adjacent to the ventricle.
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