Gene Expression Data
Assay Details
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Assay
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Reference:
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J:77124
Shinozaki K, et al., Absence of Cajal-Retzius cells and subplate neurons associated with defects of tangential cell migration from ganglionic eminence in Emx1/2 double mutant cerebral cortex. Development. 2002 Jul;129(14):3479-92
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2182348
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Gene symbol:
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Nes
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Gene name:
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nestin
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Antibody:
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clone RC2
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Detection system:
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Secondary antibody
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Assay notes:
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The authors specify that the secondary antibody was fluorescence-cojugated. The fixation for this assay was 2% paraformaldehyde and 1.35% lysine in a phosphate buffer.
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Results
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Specimen
6G:
embryonic day 16.5
(more  )
Specimen
6G:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cerebral cortex |
Present |
Single cells |
6G
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The staining is in radial glial cells.
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Specimen
6H:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx2tm1Sia/Emx2tm1Sia
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cerebral cortex |
Present |
Single cells |
6H
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Staining is in radial glial cells. The alignment of the radial fibers is disrupted in this mutant.
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Specimen
6I:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Emx1tm1Sia/Emx1tm1Sia
Emx2tm1Sia/Emx2tm1Sia
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cerebral cortex |
Present |
Single cells |
6I
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Staining is in radial glial cells. The alignment of the radial fibers is severely disrupted in this mutant.
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Analysis Tools
