Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75943
Laurikkala J, et al., Regulation of hair follicle development by the TNF signal ectodysplasin and its receptor Edar. Development. 2002 May;129(10):2541-53
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2180528
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Gene symbol:
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Lef1
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Gene name:
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lymphoid enhancer binding factor 1
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Probe:
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Lef1 cDNA3
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
7E:
embryonic day 15.0
(more  )
Specimen
7E:
(close  )
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Genetic Background:
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NMRI
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: skin |
Present |
Regionally restricted |
7E
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There is intense expression in the mesenchyme. There is also expression in the placodes of the first wave of tylotrich hairs on the back.
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Specimen
7F:
embryonic day 15.0; EdaTa/ EdaTa
(more )
Specimen
7F:
(close )
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Genetic Background:
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B6CBACa Aw-J/A-EdaTa/J-XO
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Mutant Allele(s):
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EdaTa/EdaTa
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: skin |
Present |
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7F
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The placodes have not been initiated, so expression is present throughout the ectoderm. There is also expression in the mesenchyme underlying the ectoderm.
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Specimen
7G:
embryonic day 17.0
(more )
Specimen
7G:
(close )
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Genetic Background:
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NMRI
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: skin |
Present |
Regionally restricted |
7G
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Expression is present in the placodes of the awl follicles.
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| TS25: hair follicle |
Present |
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7G
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Expression is present in the developing tylotrich hair follicles.
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Specimen
7H:
embryonic day 17.0; EdaTa/ EdaTa
(more )
Specimen
7H:
(close )
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Genetic Background:
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B6CBACa Aw-J/A-EdaTa/J-XO
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Mutant Allele(s):
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EdaTa/EdaTa
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: skin |
Present |
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7H
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There is intense expression in the placodes. There is also expression in the basal epithelium and in the mesenchyme underlying the placodes.
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Analysis Tools