Gene Expression Data
Assay Details
Assay
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Reference:
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J:54178
Huang EJ, et al., Expression of Trk receptors in the developing mouse trigeminal ganglion: in vivo evidence for NT-3 activation of TrkA and TrkB in addition to TrkC. Development. 1999 May;126(10):2191-203
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2180286
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Antibody:
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Anti-NF-M
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Detection system:
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Secondary antibody
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Assay notes:
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It is possible that another antibody (MGI:3628953) was used in this assay.
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Results
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Specimen
2A:
embryonic day 11.5
(more )
Note: Double labeled: green - Nefm; red - Ntrk3 (assay MGI:2180298).
Specimen
2A:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Nefm; red - Ntrk3 (assay MGI:2180298).
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Specimen
2C:
embryonic day 11.5
(more )
Note: Double labeled: green - Nefm; red - Ntrk2 (assay MGI:2180297).
Specimen
2C:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 11.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Carnoy's Fixative
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Embedding:
|
Paraffin
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Note:
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Double labeled: green - Nefm; red - Ntrk2 (assay MGI:2180297).
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Specimen
2E:
embryonic day 11.5
(more )
Note: Double labeled: green - Nefm; red - Ntrk1 (assay MGI:2180296).
Specimen
2E:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 11.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Nefm; red - Ntrk1 (assay MGI:2180296).
|
Specimen
2F:
embryonic day 13.5
(more )
Note: Double labeled: green - Nefm; red - Ntrk2 (assay MGI:2180297).
Specimen
2F:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 13.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
|
Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Nefm; red - Ntrk2 (assay MGI:2180297).
|
Specimen
2G:
embryonic day 13.5
(more )
Note: Double labeled: green - Nefm; red - Ntrk3 (assay MGI:2180298).
Specimen
2G:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 13.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Nefm; red - Ntrk3 (assay MGI:2180298).
|
Specimen
not shown:
embryonic day 12.5
(more )
Note: Also stained for Ntrk1 (assay MGI:2180296).
Specimen
not shown:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 12.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
|
optical section
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Fixation:
|
Carnoy's Fixative
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Embedding:
|
Paraffin
|
Note:
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Also stained for Ntrk1 (assay MGI:2180296).
|
Specimen
Table 2:
embryonic day 10.5
(more )
Note: This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
Specimen
Table 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
|
Specimen
Table 2:
embryonic day 10.5; Ntf3tm1Lfr/ Ntf3tm1Lfr
(more )
Note: This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
Specimen
Table 2:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3tm1Lfr
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Age:
|
embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: trigeminal ganglion |
Present |
|
|
The number of immunoreactive cells in the trigeminal ganglion was not significantly different from wild-type embryos.
|
Specimen
Table 2:
embryonic day 11.5
(more )
Note: This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
Specimen
Table 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
|
Specimen
Table 2:
embryonic day 11.5; Ntf3tm1Lfr/ Ntf3tm1Lfr
(more )
Note: This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
Specimen
Table 2:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: trigeminal ganglion |
Present |
|
|
The number of immunoreactive cells in the trigeminal ganglion was 58% of wild-type embryos.
|
Specimen
Table 2:
embryonic day 12.5
(more )
Note: This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
Specimen
Table 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
|
Specimen
Table 2:
embryonic day 12.5; Ntf3tm1Lfr/ Ntf3tm1Lfr
(more )
Note: This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
Specimen
Table 2:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: trigeminal ganglion |
Present |
|
|
The number of immunoreactive cells in the trigeminal ganglion was 74% of wild-type embryos.
|
Specimen
Table 2:
embryonic day 13.5
(more )
Note: This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
Specimen
Table 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
|
Specimen
Table 2:
embryonic day 13.5; Ntf3tm1Lfr/ Ntf3tm1Lfr
(more )
Note: This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
Specimen
Table 2:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: trigeminal ganglion |
Present |
|
|
The number of immunoreactive cells in the trigeminal ganglion was 31% of wild-type embryos.
|
Specimen
Table 2:
embryonic day 15.5
(more )
Note: This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
Specimen
Table 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
|
Specimen
Table 2:
embryonic day 15.5; Ntf3tm1Lfr/ Ntf3tm1Lfr
(more )
Note: This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
Specimen
Table 2:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
Mutant Allele(s):
|
Ntf3tm1Lfr/Ntf3tm1Lfr
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
Carnoy's Fixative
|
Embedding:
|
Paraffin
|
Note:
|
This data was presented in Table 2 and in a graph in Fig. 4. Some data was reprinted from an earlier publication.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: trigeminal ganglion |
Present |
|
|
The number of immunoreactive cells in the trigeminal ganglion was 30% of wild-type embryos.
|
|