Gene Expression Data
Assay Details
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Assay
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Reference:
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J:55428
Chapouton P, et al., The role of Pax6 in restricting cell migration between developing cortex and basal ganglia. Development. 1999 Dec;126(24):5569-79
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2179925
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Gene symbol:
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Calb2
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Gene name:
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calbindin 2
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Antibody:
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anti-calretinin
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Detection system:
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Secondary antibody coupled to Cy3
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Assay notes:
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specimens were embedded in 3% agarose and vibratome sections (100 microns) were cut. The data shown are schematics of counts of the quantitative analyses. All immunopositive cells in a single field of view were counted in the lateral cortex, immediately ventral to the sulcus. All cells in the cortical plate of the dorsal cortex were counted from the sulcus to the fold of the medial cortex.
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Results
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Specimen
7 wt:
embryonic day 15.5
(more  )
Specimen
7 wt:
(close  )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
Regionally restricted |
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Positive cells in the lateral and dorsal cortex.
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Specimen
7 Sey:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
Regionally restricted |
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Positive cells in the lateral and dorsal cortex with more positive cells in the lateral cortex as compared to wild-type embryos.
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Analysis Tools
