Gene Expression Data
Assay Details
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Assay
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Reference:
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J:55428
Chapouton P, et al., The role of Pax6 in restricting cell migration between developing cortex and basal ganglia. Development. 1999 Dec;126(24):5569-79
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2179921
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Gene symbol:
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Calb1
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Gene name:
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calbindin 1
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Antibody:
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anti-Calbindin D28k (CB-38)
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Detection system:
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Secondary antibody coupled to Cy3
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Assay notes:
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specimens were embedded in 3% agarose and vibratome sections (100 microns) were cut. The data shown in Figure 7 are schematics with counts of the quantitative analyses. All immunopositive cells in a single field of view were counted in the lateral cortex, immediately ventral to the sulcus. All cells in the cortical plate of the dorsal cortex were counted from the sulcus to the fold of the medial cortex.
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Results
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Specimen
6A:
embryonic day 15.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
Regionally restricted |
6A
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Positive cells in the lateral cortex.
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Specimen
6B:
embryonic day 15.5
(more )
Specimen
6B:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
Regionally restricted |
6B
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Positive cells in the lateral cortex.
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Specimen
6C:
embryonic day 15.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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Specimen
6D:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
Regionally restricted |
6D
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More positive cells in the lateral cortex as compared to wild-type embryos.
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Specimen
6E:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
Regionally restricted |
6E
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More positive cells in the lateral cortex as compared to wild-type embryos.
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Specimen
6F:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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Specimen
7 wt:
embryonic day 15.5
(more )
Specimen
7 wt:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
Regionally restricted |
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Positive cells in the lateral and dorsal cortex.
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Specimen
7 Sey:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
Regionally restricted |
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More positive cells in the lateral cortex as compared to wild-type.
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| TS23: cortical plate |
Present |
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Similar numbers of positive cells in the cortical plate of the dorsal cortex.
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Specimen
7 wt:
embryonic day 17.5
(more )
Specimen
7 wt:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cerebral cortex |
Present |
Regionally restricted |
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Positive cells in the lateral and dorsal cortex.
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Specimen
7 Sey:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cerebral cortex |
Present |
Regionally restricted |
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More positive cells in the lateral and dorsal cortex, cortical plate, as compared to wild-type embryos.
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| TS25: cortical plate |
Present |
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Analysis Tools