Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:74631
Tian E, et al., Otx2 is required to respond to signals from anterior neural ridge for forebrain specification. Dev Biol. 2002 Feb 15;242(2):204-23
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2179006
|
|
Gene symbol:
|
Fgf8
|
|
Gene name:
|
fibroblast growth factor 8
|
|
Probe:
|
Fgf8 cDNA13
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
4I:
embryonic day 9.5
(more  )
Specimen
4I:
(close  )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4J:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Otx2tm1Sia/Otx2tm4Sia
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: telencephalon |
Present |
|
4J
|
Expression throughout entire rostral region.
|
Specimen
not shown:
embryonic day 8.0
(more )
Note: 4 somite stage.
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age and stage of embryo determined from somite number.
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
4 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: surface ectoderm |
Present |
|
|
Authors report expression commences in nonneural ectoderm around the 4-somite stage.
|
Specimen
not shown:
embryonic day 8.0
(more )
Note: 6 somite stage.
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age and stage of embryo determined from somite number.
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
6 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural ectoderm |
Present |
|
|
Expression in neurectoderm following the 6-somite stage.
|
Specimen
8I:
embryonic day 8.5
(more )
Note: 8 somite stage.
Specimen
8I:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age and stage of embryo determined from somite number.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
8 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Strong |
Regionally restricted |
8I
|
Expression throughout entire rostral region, including nonneural ectoderm.
|
| TS13: surface ectoderm |
Strong |
Regionally restricted |
8I
|
Expression throughout entire rostral region, including nonneural ectoderm.
|
Specimen
8J:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Otx2tm1Sia/Otx2tm4Sia
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age and stage of embryo determined from somite number.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
8 somite stage.
|
Specimen
8K:
embryonic day 8.5
(more )
Note: 8 somite stage.
Specimen
8K:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age and stage of embryo determined from somite number.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
8 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural ectoderm |
Present |
Regionally restricted |
8K
|
Expression throughout entire rostral region, including nonneural ectoderm.
|
| TS13: surface ectoderm |
Present |
Regionally restricted |
8K
|
Expression throughout entire rostral region, including nonneural ectoderm.
|
Specimen
8L:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Otx2tm1Sia/Otx2tm4Sia
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age and stage of embryo determined from somite number.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
8 somite stage.
|
Specimen
8M:
embryonic day 8.5
(more )
Note: 12 somite stage.
Specimen
8M:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age and stage of embryo determined from somite number.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
12 somite stage.
|
Specimen
8N:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Otx2tm1Sia/Otx2tm4Sia
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age and stage of embryo determined from somite number.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
12 somite stage.
|
|
Analysis Tools