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Gene Expression Data
Assay Details
Assay
Reference: J:73071 Long F, et al., Genetic manipulation of hedgehog signaling in the endochondral skeleton reveals a direct role in the regulation of chondrocyte proliferation. Development. 2001 Dec;128(24):5099-108
Assay type: RNA in situ
MGI Accession ID: MGI:2178828
Gene symbol: Ptch1
Gene name: patched 1
Probe: Ptc 1 probe2
Probe preparation: Antisense labelled with S35 RNA
Results
Specimen 3 E14.5 WT: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: tibia cartilage condensation Present 3_Ptc1 E14.5 WT Expression was present in the proliferating chondrocytes.
Specimen 3 E14.5 Cre15;Smon/c: embryonic day 14.5; Smotm1Amc/Smotm2Amc, Tg(Col2a1-cre)15Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: tibia cartilage condensation Present Single cells 3_Ptc1 E14.5 Cre15;Smon/c Expression was detected in a few chondrocytes.
TS22: lower leg rest of mesenchyme Present Regionally restricted 3_Ptc1 E14.5 Cre15;Smon/c Expression in the perichondrium was markedly enhanced in the conditional mutant, and its expression domain expanded towards the epiphysis.
Specimen 3 E14.5 Cre10;Smon/c: embryonic day 14.5; Smotm1Amc/Smotm2Amc, Tg(Col2a1-cre)10Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: tibia cartilage condensation Absent 3_Ptc1 E14.5 Cre10;Smon/c The mutant completely lacked expression in immature proliferating chondrocytes.
TS22: lower leg rest of mesenchyme Present Regionally restricted 3_Ptc1 E14.5 Cre10;Smon/c Expression in the perichondrium was markedly enhanced in the conditional mutant, and its expression domain expanded towards the epiphysis.
Specimen not shown: embryonic day 14.5; Smotm1Amc/Smotm2Amc, Tg(Col2a1-cre)3Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS22: tibia cartilage condensation Present Single cells Expression was detected in a few chondrocytes.
TS22: lower leg rest of mesenchyme Present Regionally restricted Expression in the perichondrium was markedly enhanced in the conditional mutant, and its expression domain expanded towards the epiphysis.
Specimen 3 E18.5 WT: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: tibia Present 3_Ptc1 E18.5 WT Expression was present in the chondrocytes.
Specimen 3 E18.5 Cre15;Smon/c: embryonic day 18.5; Smotm1Amc/Smotm2Amc, Tg(Col2a1-cre)15Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: tibia Present Single cells 3_Ptc1 E18.5 Cre15;Smon/c Expression was detected in a few chondrocytes.
TS26: lower leg mesenchyme Present Regionally restricted 3_Ptc1 E18.5 Cre15;Smon/c Expression in the perichondrium was markedly enhanced in the conditional mutant, and its expression domain expanded towards the epiphysis.
Specimen 3 E18.5 Cre10;Smon/c: embryonic day 18.5; Smotm1Amc/Smotm2Amc, Tg(Col2a1-cre)10Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: tibia Absent 3_Ptc1 E18.5 Cre10;Smon/c The mutant completely lacked expression in immature proliferating chondrocytes.
TS26: lower leg mesenchyme Present Regionally restricted 3_Ptc1 E18.5 Cre10;Smon/c Expression in the perichondrium was markedly enhanced in the conditional mutant, and its expression domain expanded towards the epiphysis.
Specimen not shown: embryonic day 18.5; Smotm1Amc/Smotm2Amc, Tg(Col2a1-cre)3Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS26: tibia Absent Expression was lost in chondrocytes by E18.5.
TS26: lower leg mesenchyme Present Regionally restricted Expression in the perichondrium was markedly enhanced in the conditional mutant, and its expression domain expanded towards the epiphysis.
Specimen 6B WT: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: tibia-fibula pre-cartilage condensation Present 6B WT Expression was present in proliferating chondrocytes.
TS21: lower leg rest of mesenchyme Present Regionally restricted 6B WT Expression was present in perichondrium.
Specimen 6B Cre10;Smon/c: embryonic day 13.5; Smotm1Amc/Smotm2Amc, Tg(Col2a1-cre)10Amc/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: tibia-fibula pre-cartilage condensation Absent 6B Cre10;Smon/c Expression was absent in proliferating chondrocytes.
TS21: lower leg rest of mesenchyme Present Regionally restricted 6B Cre10;Smon/c Expression in the perichondrium was unaffected.
Specimen 7B: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: tibia Present 7B Ptc-1 WT E16.5 Expression was present in the chondrocytes.
Specimen 7C: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: tibia Present 7C E16.5 Ptc-1 WT Expression was present in the chondrocytes.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory