Gene Expression Data
Assay Details
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Assay
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Reference:
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J:29279
Patapoutian A, et al., Disruption of the mouse MRF4 gene identifies multiple waves of myogenesis in the myotome. Development. 1995 Oct;121(10):3347-58
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2178649
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Gene symbol:
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Actn2
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Gene name:
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actinin alpha 2
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Antibody:
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anti alpha-actinin (EA-53)
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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This antibody is specific for alpha-skeletal and alpha-cardiac muscle actinins and does not react to non-skeletal muscle elements. It is possible that other alpha-actinins are recognized as well.
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Results
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Specimen
4A:
embryonic day 11.0
(more  )
Specimen
4A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk somite |
Present |
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4A
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Expression in the somites at the forelimb level.
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Specimen
4B:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Myf6tm1Wb/Myf6tm1Wb
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk dermomyotome |
Present |
Regionally restricted |
4B
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Expression in the somites at the forelimb level is reduced to the dorsal, earlier forming myogenic cells.
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Specimen
4G:
embryonic day 11.0
(more )
Specimen
4G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk somite |
Present |
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4G
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Expression in the somites at the hindlimb level.
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Specimen
4H:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Myf6tm1Wb/Myf6tm1Wb
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Age:
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embryonic day 11.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk dermomyotome |
Present |
Regionally restricted |
4H
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Expression in the somites at the hindlimb level is reduced to the dorsal, earlier forming myogenic cells.
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Specimen
6E:
embryonic day 18.5
(more )
Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Myf6tm1Wb/Myf6tm1Wb
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: intercostal muscle |
Present |
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6F
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Expression in this mutant is the same as in wild-type animals but shows that these mice have abnormal muscle formation between the ribs.
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Analysis Tools