Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75954
Dunwoodie SL, et al., Axial skeletal defects caused by mutation in the spondylocostal dysplasia/pudgy gene Dll3 are associated with disruption of the segmentation clock within the presomitic mesoderm. Development. 2002 Apr;129(7):1795-806
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2178633
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Gene symbol:
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Mesp2
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Gene name:
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mesoderm posterior 2
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Probe:
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MesP2 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A,B:
embryonic day 9.5; Dll3tm1Rbe/Dll3 +
(more  )
Specimen
5A,B:
(close  )
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Genetic Background:
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involves: 129P2/Ola * C57BL/6
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Mutant Allele(s):
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Dll3tm1Rbe/Dll3+
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
5B
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5A
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Anterior boundary of expression approximately 1 somite width caudal to the posterior boundary of the last formed somite.
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Specimen
5C,D:
(close )
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Genetic Background:
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involves: 129P2/Ola * C57BL/6
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Mutant Allele(s):
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Dll3tm1Rbe/Dll3tm1Rbe
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
5D
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5C
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Expression boundary approximately 3 somite widths posterior to the last formed somite.
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Specimen
5E,F:
(close )
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Genetic Background:
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involves: 129P2/Ola * C57BL/6
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Mutant Allele(s):
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Dll3tm1Rbe/Dll3tm1Rbe
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Bouin's Fixative
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: tail unsegmented mesenchyme |
Present |
Regionally restricted |
5E
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5F
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No epithelial somites were detected in this -/- embryo; authors state the band of expression is an equivalent position to that seen in the +/- embryo.
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Analysis Tools