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Gene Expression Data
Assay Details
Assay
Reference: J:74785 Drewell RA, et al., Novel conserved elements upstream of the H19 gene are transcribed and act as mesodermal enhancers. Development. 2002 Mar;129(5):1205-13
Assay type: RT-PCR
MGI Accession ID: MGI:2178390
Gene symbol: Rr563
Gene name: regulatory region 563
Probe: Huc-pA, Huc-pB
Probe preparation: Double stranded labelled with P32 DNA
Assay notes: The RT-PCR products were electrophoresed, blotted, and then hybridized using a probe (MGI:2178375) that recognizes Huc1, Huc2, and the sequence intervening between them.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 1.86 kb (a) Amount Genetic BackgroundMutant Allele(s)Sex
g 1-1 + Control
g 1-1 - Control
g 2-2 + Control
g 2-2 - Control
g 1-2 + Control
g 1-2 - Control
RT 1-1 + Lane not used in this assay
RT 1-1 - Control
RT 2-2 + Lane not used in this assay
RT 2-2 - Control
RT 1-2 + E13.5 (b) TS21: embryo Absent Not Specified; total RNA Not Specified Not Specified
RT 1-2 - Control
Notes:
(a) The primers were designed to amplify across the two genes only if they are part of the same transcriptional unit.
(b) Age of embryo at noon of plug day not specified in reference.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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