Igf2 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:75952 Davies K, et al., Disruption of mesodermal enhancers for Igf2 in the minute mutant. Development. 2002 Apr;129(7):1657-68
Assay type:	Northern blot
MGI Accession ID:	MGI:2178264
Gene symbol:	Igf2
Gene name:	insulin-like growth factor 2

Probe:	Probe 6
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	4.7 kb	3.8 kb	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Mnt^p Brain	P newborn	TS27: brain	Not Specified	Not Specified	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	\Minute/\Minute⁺	Not Specified	The minute allele was inherited via paternal transmission.
Mnt^p Tongue	P newborn	TS27: tongue	Present (a)	Present (a)	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	\Minute/\Minute⁺	Not Specified	The minute allele was inherited via paternal transmission.
Mnt^p Heart	P newborn	TS27: heart	Absent	Absent	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	\Minute/\Minute⁺	Not Specified	The minute allele was inherited via paternal transmission.
Mnt^p Lung	P newborn	TS27: lung	Absent	Absent	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	\Minute/\Minute⁺	Not Specified	The minute allele was inherited via paternal transmission.
Mnt^p Liver	P newborn	TS27: liver	Present (b)	Present (b)	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	\Minute/\Minute⁺	Not Specified	The minute allele was inherited via paternal transmission.
Mnt^p Kidney	P newborn	TS27: metanephros	Absent	Absent	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	\Minute/\Minute⁺	Not Specified	The minute allele was inherited via paternal transmission.
Mnt^p Intestine	P newborn	TS27: intestine	Present (c)	Present (c)	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	\Minute/\Minute⁺	Not Specified	The minute allele was inherited via paternal transmission.
Mnt^p Muscle	P newborn	TS27: musculature	Present (d)	Present (d)	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus	\Minute/\Minute⁺	Not Specified	The minute allele was inherited via paternal transmission.
Brain	P newborn	TS27: brain	Not Specified	Not Specified	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus		Not Specified
Tongue	P newborn	TS27: tongue	Present	Present	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus		Not Specified
Heart	P newborn	TS27: heart	Present	Present	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus		Not Specified
Lung	P newborn	TS27: lung	Present	Present	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus		Not Specified
Liver	P newborn	TS27: liver	Present	Present	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus		Not Specified
Kidney	P newborn	TS27: metanephros	Present	Present	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus		Not Specified
Intestine	P newborn	TS27: intestine	Present	Present	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus		Not Specified
Muscle	P newborn	TS27: musculature	Present	Present	10 µg; total RNA	involves: 101/H * C3H/HeH * C57BL/6J * CBA/Ca * M. spretus		Not Specified

Notes:
(a) Authors report substantially reduced levels (35% of wild type).
(b) Authors report normal levels.
(c) Authors report moderately reduced levels (71% of wild type).
(d) Authors report substantially reduced levels (62% of wild type).

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